Abstract
Two facultative anoxygenic photoautotrophic cyanobacteria, Oscillatoria limnetica and Aphanothece halophytica were found capable of CO2 photoassimilation using molecular hydrogen as electron donor in a photosystem I driven reaction. A. halophytica was also capable of evolving hydrogen from Na-dithionite reduced methylviologen in a light independent reaction.
Abbreviations
- DCMU:
-
3-(3,4-dichlorophenyl)-1,1-dimethylurea
- DSPD:
-
Disallcylidenepropanediamine
- FCCP:
-
Carbonylcyanide p-trifluoromethoxyphenyl hydrazone
- Tricine:
-
N-tris(hydroxy methyl)-methylglycine
References
Arnon, D. I.: Copper enzymes in isolated chloroplasts; polyphenoloxidase in Beta vulgaris. Plant Physiol. 24, 1–15 (1949)
Avron, M., Gibbs, M.: Properties of phosphoribulokinase of whole chloroplasts. Plant Physiol. 53, 136–139 (1974)
Ben Amotz, A., Gibbs, M.: H2 metabolism in photosynthetic organisms. II. Light dependent H2 evolution by preparations from Chlamydomonas scenedesmus and spinach. Biochem. Biophys. Res. Comm. 64, 355–359 (1975)
Benemann, J. R., Weare, N. M.: Hydrogen evolution by nitrogenfixing Anabaena cylindrica cultures. Science 184, 174–175 (1974)
Bothe, H., Tennigkeit, J., Eisenberger, G., Yates, M. G.: The hydrogenase-nitrogenase relationship in the blue-green alga Anabaena cylindrica. Planta (Berl.) 133, 237–242 (1977)
Cohen, Y., Jørgensen, B. B., Padan, E., Shilo, M.: Sulphide-dependent anoxygenic photosynthesis in the cyanobacterium Oscillatoria limnetica. Nature (London) 257, 489–492 (1975a)
Cohen, Y., Padan, E., Shilo, M.: Facultative anoxygenic photosynthesis in the cyanobacterium Oscillatoria limnetica. J. Bacteriol 123, 855–861 (1975a)
Gaffron, H.: Carbon dioxide reduction with molecular hydrogen in green algae. Am. J. Bot. 27, 273–283 (1940)
Gaffron, H.: On dating stages in photochemical evolution. In: Horizons in biochemistry (M. Kasha, B. Pullman, eds.), pp. 59–89. New York: Academic Press 1962
Garlick, S., Oren, A., Padan, E.: Occurrence of facultative anoxygenic photosynthesis among filamentous and unicellular cyanobacteria. J. Bacteriol. 129, 623–629 (1977)
Gest, H.: Energy conversion and generation of reducing power in bacterial photosynthesis. Adv. Microbiol. Physiol. 7, 243–282 (1972)
Gray, C. T., Gest, H.: Biological formation of molecular hydrogen. Science 148, 186–191 (1965)
Hillmer, P., Gest, H.: H2 metabolism in the photosynthetic bacterium Rhodopseudomonas capsulata: H2 production by growing cultures. J. Bacteriol. 129, 724–731 (1977)
Kessler, E.: Hydrogenase, photoreduction and anaerobic growth. In: Algal physiology and biochemistry (W. D. P. Stewart, ed.), pp. 456–473. Oxford: Blackwell 1974
Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J.: Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265–275 (1951)
Oren, A., Padan, E., Avron, M.: Quantum yields for oxygenic and anoxygenic photosynthesis in the cyanobacterium Oscillatoria limnetica. Proc. Natl. Acad. Sci. U.S.A. 74, 2152–2156 (1977)
Schlegel, H. G.: Mechanisms of chemo-autotrophy. In: Marine ecology, Vol. 2, Part I (O. Kinne, ed.), pp. 9–60. London: Wiley 1975
Schopf, J. W.: Paleobiology of the Precambrian: the age of the blue-green algae. In: Evolutionary biology, vol. 7 (T. Dobzhansky, A. R. Hecht, W. C. Steere, eds.), pp. 1–43. New York: Plenum Press 1974
Tel-Or, E., Luijk, L. W., Packer, L.: An inducible hydrogenase in cyanobacteria enhances N2 fixation. FEBS Letters 78, 49–52 (1977)
Ward, B., Myers, J.: Photosynthetic properties of permeaplasts of Anacystis. Plant Physiol. 50, 547–550 (1972)
Weissman, J. C., Benemann, J. R.: Hydrogen production by nitrogen starved cultures of Anabaena cylindrica. Appl. Environ. Microbiol. 33, 123–131 (1977)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Belkin, S., Padan, E. Hydrogen metabolism in the facultative anoxygenic cyanobacteria (blue-green algae) Oscillatoria limnetica and Aphanothece halophytica . Arch. Microbiol. 116, 109–111 (1978). https://doi.org/10.1007/BF00408741
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00408741