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Heterotopic heart transplantation alters high-energy phosphate metabolism irrespective of cardiac allograft rejection

  • Part II: Cardiac Hypertrophy and Failure
  • Published:
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Abstract

This study was undertaken to validate the potential of 31P magnetic resonance spectroscopy (MRS) as a noninvasive alternative for transvenous endomyocardial biopsy in detecting cardiac allograft rejection. Donor hearts from either Lewis rats (L) or Brown-Norway rats (BN) were transplanted into the neck of L rats resulting in a non-rejecting group L-L and a rejecting group L-BN. L-L and L-BN rats were serially studied by means of 31P MRS from postoperatine day 1–8. In addition, rejection was confirmed by histology. A similar, marked decrease in phosphocreative/β-adenosinetriphosphate (PCr/ATP) ratio from day 1–3 was observed in both L-L and L-BN hearts. This ratio levelled off on postoperative day 3 and remained depressed on subsequent postoperative days in both groups, although histology showed an increase in the severity of rejection in L-BN. However, the PCr signal/noise ratio in L-BN started to decrease after day 4, coinciding with the histologic evidence of severe rejection (score IV), whereas in L-L hearts (score 0) this ratio remained unaltered until day 8. Since high-energy phosphate metabolism is affected by the unloaded status of the heterotopically transplanted heart, irrespective of rejection, the PCr/ATP ratio appears not to be a specific marker for the detection of acute rejection in this model. In contrast, the PCr S/N ratio appears to be a specific and sensitive marker of acute rejection, but only in a late, severe stage.

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Authors and Affiliations

  1. Interuniversity Cardiology Institute of The Netherlands, University Hospital, Utrecht, The Netherlands

    J. Onno van Dobbenburgh, Carina Kasbergen, Tom J. C. Ruigrok & Cees J. A. van Echteld

  2. Department of Cardiology, Heart Lung Institute, University Hospital, Utrecht, The Netherlands

    J. Onno van Dobbenburgh, Carina Kasbergen, Tom J. C. Ruigrok & Cees J. A. van Echteld

  3. Department Of Pathology, University Hospital, Utrecht, The Netherlands

    Piet J. Slootweg

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  1. J. Onno van Dobbenburgh
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  2. Carina Kasbergen
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  3. Piet J. Slootweg
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  4. Tom J. C. Ruigrok
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  5. Cees J. A. van Echteld
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van Dobbenburgh, J.O., Kasbergen, C., Slootweg, P.J. et al. Heterotopic heart transplantation alters high-energy phosphate metabolism irrespective of cardiac allograft rejection. Mol Cell Biochem 163, 247–252 (1996). https://doi.org/10.1007/BF00408665

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