Summary
Colchicine at 5 and 10 p.p.m. increased both phosphorus uptake and incorporation into organic forms (nucleoproteins or other simpler organophosphorus compounds). Continuous supply of colchicine at 20 p.p.m., almost checked phosphorus uptake during the second 24 hours of the experiment.
The absorbed nitrates were utilised through the classical reduction steps. It appears also that colchicine had an inhibitory effect on the nitrite reductase that increased by increasing the concentration of the drug. Continuous supply of colchicine at its highest concentration (20 p.p.m.) checked completely the protein building during the second 24 hours of the experiment, though nitrate absorption continued; a phenomenon that caused the accumulation, in the tissue medium systems, of large amounts of peptide nitrogen.
The mechanism of nitrate reduction as affected by colchicine treatment was fully discussed.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Clark, H. E.: Effect of ammonia and nitrate nitrogen on the composition of tomato plants. Plant Physiol. 11, 5 (1936).
Davis, E. A.: Nitrate reduction by Chlorella. Plant Physiol. 28, 539 (1953).
Delwiche, C. C.: Assimilation of ammonia and nitrate ions by tomato plants. J. Biochem. 189, 167 (1951).
Evans, H. J., and A. Nason: Pyridine nucleotide nitrate reductase from extracts of higher plants. Plant Physiol. 28, 233 (1953).
Fewson, C. A., and D. J. D. Nicholas: Utilisation of nitrate by microorganisms. Nature (Lond.) 190, 2 (1961).
Friedman, N. B.: Effect of chemotherapy and irradiation therapy on the differentiation of experimental tumors. Cancer 11, 1060 (1958).
Hattori, A.: Light induced reduction of nitrate, nitrite and hydroxylamine in a blue green alga Anabaena cylindrica. Plant and Cell Physiol. 3, 355 (1962a).
—: Adaptive character of nitrate reducing systems in Anabaena cylindrica. Plant and Cell Physiol. 3, 371 (1962b).
Hattori, A.: Effect of hydrogen on nitrite reduction by Anabaena cylindrica. In: Studies on microalgae and photosynthetic bacteria. Special Issue of Plant and Cell Physiol., p. 485 (1963).
Kessler, E.: Role of photochemical processes in the reduction of nitrate by green plants. Nature (Lond.) 176, 1069 (1955).
—: Untersuchungen zum Problem der photochemischen Nitratreduktion in Grünalgen. Planta (Berl.) 49, 505 (1957a).
—: Contribution to the problem of photochemical nitrate reduction. In: Research in photosynthesis. by H. Gaffron et al. p. 250–556. New York: Interscience Publication, Inc. 1957b.
—: Reduction of nitrates by green algae. Symp. Soc. exp. Biol. 13, 87 (1959).
—: Über der Wirkung von Arsenat auf Nitratreduktion, Atmung und Photosynthese von Grünalgen. Planta (Berl.) 55, 512 (1960).
Lang, K., G., Siebert, and W. Estelmann: Inhibition of adenosine triphosphatase by colchicine. Experientia (Basel). 7, 379 (1951).
—— and H. Oswald: Inhibition of desoxyribonucleotide splitting enzymes by colchicine. Experientia (Basel) 5, 449 (1949).
Mendel, J. L., and D. W. Visser: Studies on nitrate reduction in higher plants. Arch. Biochem. 32, 158 (1951).
Naguib, M. I.: Effect of colchicine on the nitrogen metabolism and fate of phosphates absorbed during the formation of fungal felts of Cunninghamella sp. Arch. Mikrobiol. 47, 154 (1963).
Nason, A.: Enzymatic pathways of nitrate, nitrite and hydroxylamine metabolism. Bact. Rev. 26, 16 (1962).
—, and H. Takahashi: Inorganic nitrogen metabolism. Z. allg. Mikrobiol. 1, 341 (1958).
Pucher, G. W., C. S. Laevenworth, W. D. Ginter, and H. B. Vickery: Studies in the metabolism of crassulacean plants. Effects upon the composition of Bryophyllum calycinum of the form of nitrogen supplied. Plant Physiol. 22, 205 (1947).
Said, H., and K. Naguib: Effect of 2:4-Dichlorophenoxyacetic acid on absorption and assimilation of sucrose by Fusarium moniliforme, Sheldon. Bull. Fac. Sci. Cairo Univ. 38, 49 (1962).
Salama, A. M., and M. I. Naguib: Effect of colchicine on the carbohydrate metabolism of mycelial felts of Cunnighamella sp. Arch. Mikrobiol. 46, 296 (1963).
—— Colchicine after effects on the absorption and utilisation of sucrose by Cunninghamella sp. Arch. Mikrobiol. 48, 185 (1964)
Sideris, C. P., B. H. Krauss, and H. Y. Young: Assimilation of ammonia and nitrate nitrogen from solution cultures by roots of Pandanus veitchii Hort and the distribution of the various nitrogen fractions and sugars in the stele and cortex. Plant Physiol. 12, 899 (1937).
———: Assimilation of ammonia and nitrates by pineapple plants grown on nutrient solutions and its effects on the nitrogen and carbohydrate constituents. Plant Physiol. 13, 489 (1938).
Street, H. E., A. E. Kenyon, and G. M. Watson: The assimilation of ammonia and nitrate nitrogen by detatched potato sprouts. Ann. appl. Biol. 33, 369 (1946).
Tiedjens, V. A. and M. A. Blake: Factors affecting the use of nitrate and ammonium nitrate by apple trees. N. J. Agr. Exptal. Stat. Bull. No. 547 (1932).
Van Niel, C., M. B. Allen, and B. E. Wright: On the photochemical reduction of nitrate by algae. Biochem. biophys. Acta (Amst.) 12, 67 (1953).
Vladimirov, A. V.: Variation in the citric acid and nicotine contents of Makhorka leaves (Nicotiana rustica) in relation to ammonia and nitrate assimilation of plants. Chemisation Socialistic Agricult. U.S.S.R. 8, 35 (1939).
—: Influence of nitrogen source in the formation of reduced and oxidised compounds in plants. Soil Sci. 60, 265 (1945).
Wadleigh, C. H., and J. W. Shive: Base content of corn plants as influenced by pH of substrate and form of nitrogen supply. Soil Sci. 47, 273 (1939).
Wang, D. Y., A. L. Greenbaum, and R. D. Harkness: The effect of colchicine on the acid soluble ribonucleotides of normal and regenerating rat liver. Biochem. J. 86, 62 (1963).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Naguib, M.I., Salama, A.M. Colchicine after-effects on the absorption and utilisation of phosphates and nitrates by mycelial felts of Cunninghamella sp.. Archiv. Mikrobiol. 48, 222–238 (1964). https://doi.org/10.1007/BF00408593
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00408593