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Fettsäuregehalte in Lichtkulturen von Rhodospirillum rubrum während der Thylakoidmorphogenese

Contents of fatty acids in light cultures of Rhodospirillun rubrum during the synthesis of thylakoids

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Summary

The correlation between the formation of the intracytoplasmic membrane system and the content of fatty acids and lipid phosphorus in cells of Rhodospirillum rubrum is investigated. After induction of thylakoid synthesis by transferring aerobic dark grown cells into anaerobic light conditions, the content of fatty acids and lipid phosphorus per protein of the cells remains unchanged. The same result is found if the increase of thylakoid content is induced by lowering the light intensity from moderate (4000 lux) to low (400 lux) light intensity under anaerobic conditions. The constant level of lipids during the morphogenesis of thylakoids is not conditioned by a change of the protein content of the cells. The ratio protein to volume unit of wet packed bacteria is not changed during the synthesis of thylakoids. Crude cell wall and membrane fractions are isolated from light and dark grown cells. The wall fraction from dark aerobic grown cells contains more fatty acids than that of anaerobic light grown cells. Label from (2-14C)-acetate is rapidly incorporated by growing cells cultivated under moderate light intensity. After transfer to low light conditions acetate incorporation and growth stop immediately. If aerobic dark grown cells are transferred to semiaerobic light conditions the content of fatty acids and lipid phosphorus in the cells is increased by 70%. The results are discussed from the view that a great part of the fatty acids of the cells is localized in the cell wall and that this amount can be altered in coordination with the membrane content of the cells.

Zusammenfassung

Der Zusammenhang zwischen der Ausbildung des intracytoplasmatischen Membransystems und dem Gehalt der Zellen an Fettsäuren und Lipid-Phosphor wird an Rhodospirillum rubrum untersucht. Trotz Steigerung des Membrangehaltes nach der Überführung aerober thylakoidfreier Dunkelzellen in anaerobe Lichtbedingungen bleibt der Gehalt an Fettsäuren und Lipid-Phosphor pro Gesamtzellprotein unverändert. Das gleiche Resultat ergibt sich, wenn eine Steigerung des Thylakoidgehaltes im System anaerob Normallicht (4000 Lux) → anaerob Schwachlicht (400 Lux) hervorgerufen wird. Dieses Resultat eines konstanten Fettsäurespiegels wird nicht durch eine Änderung des Bezugsystems Protein erzielt. Der Quotient Proteingehalt pro Volumen dichtgepackter Bakterien bleibt während der Membransynthese konstant. Aus Licht- und Dunkelzellen werden eine Rohmembranund eine Rohzellwandfraktion angereichert. Die Wandfraktion aus aeroben Dunkelzellen enthält einen höheren Anteil der Gesamtfettsäuren der Zelle als die Wandfraktion aus anaeroben Lichtzellen. (2-14C)-Acetat wird von wachsenden Zellen unter Normallichtbedingungen (4000 Lux) in hoher Rate eingebaut. Nach der Umschaltung auf Schwachlicht (400 Lux) wird diese Aufnahme zugleich mit dem Wachstum gestoppt. Werden aerobe Dunkelzellen in semiaerobe Lichtbedingungen über-führt, wird der Gehalt an Fettsäuren und Lipid-Phosphor um 70% erhöht. Die Ergebnisse werden im Rahmen der Hypothese diskutiert, daß ein großer Teil der Zellfettsäuren in der Wand lokalisiert ist und daß dieser Anteil koordiniert mit dem Membrangehalt verändert werden kann.

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Authors and Affiliations

  1. Lehrstuhl für Mikrobiologie, Institut für Biologie II der Universität Freiburg i.Br., Freiburg i.Br., Deutschland

    Joachim Schröder & Gerhart Drews

  2. Department of Microbiology, University of Minnesota, 1060 Mayo Memorial Building, 55455, Minneapolis, Minn., USA

    Joachim Schröder

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  1. Joachim Schröder
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  2. Gerhart Drews
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Schröder, J., Drews, G. Fettsäuregehalte in Lichtkulturen von Rhodospirillum rubrum während der Thylakoidmorphogenese. Archiv. Mikrobiol. 69, 20–33 (1969). https://doi.org/10.1007/BF00408559

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