Abstract
Bacillus licheniformis NCIB 6346 was grown under phosphate or magnesium limitation in continuous culture and forward and reverse transitions carried out to examine their effects upon extracellular enzyme production. Analysis of the observed kinetics allowed a distinction to be made between the effects of phenotypic variation and of genetic selection upon exoenzyme production. Furthermore, increases in the phosphate concentration (0.3–30 mM) were directly related to increased phenotypic expression of alpha-amylase. By contrast, penicillinase production was reduced under these conditions but analysis revealed that its phenotypic expression was only indirectly influenced by the concentrations of either magnesium or phosphate present during the transitions.
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Lancaster, M.J., Keevil, C.W., Ellwood, D.C. et al. Effect of environmental phosphate concentration on protein export by Bacillus licheniformis NCIB 6346. Arch. Microbiol. 150, 547–551 (1988). https://doi.org/10.1007/BF00408247
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DOI: https://doi.org/10.1007/BF00408247