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Core substructure in Mastigocladus laminosus phycobilisomes

I. Microheterogeneity in two of three allophycocyanin core complexes

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Abstract

Three allophycocyanin complexes were separated by gel electrophoresis, isoelectric focusing and ion exchange chromatography from a low molecular fraction (Mr 100–150000) of partially dissociated phycobilisomes of Mastigocladus laminosus: A. (αAPβAP); B. (α*APα2 APβ2 APβ*AP) · L 10C ; and C. (α*APαAPBαAPβ2 APβ*AP) · L 10C . According to their fluorescence emission maximum at room temperature the complexes A., B. and C. are designated AP 660, AP 664 and AP 680. The different subunits of the AP complexes have apparent molecular weights of Mr 18500 α*AP, 18200 βAPB, 18000 αAP, 17000 βAP and 16500 β*AP. This hitherto unrecognized microheterogeneity within the AP subunits of complexes B. and C. of Mastigocladus laminosus phycobilisomes could also be demonstrated and confirmed with the two phycocyanin complexes PC 642 and PC 646. PC 642 is characterized by a L 11R linker polypeptide.

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Abbreviations

AP:

allophycocyanin

PC:

phycocyanin

PEC:

phycoerythrocyanin

PE:

phycoerythrin

PAGE:

polyacrylamide gel electrophoresis

IEF:

isoelectric focusing

pI:

isoelectric point

Mr :

apparent molecular weight

TMED:

tetramethylethylenediamine

APS:

ammonium persulphate

SDS:

sodium dodecylsulphate

O.D.:

optical density

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  1. Fachbereich Biologie der Universität Marburg, D-3550, Marburg, Federal Republic of Germany

    Wolfgang Reuter & Werner Wehrmeyer

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  1. Wolfgang Reuter
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  2. Werner Wehrmeyer
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Reuter, W., Wehrmeyer, W. Core substructure in Mastigocladus laminosus phycobilisomes. Arch. Microbiol. 150, 534–540 (1988). https://doi.org/10.1007/BF00408245

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  • DOI: https://doi.org/10.1007/BF00408245

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