Abstract
Shikonin isovalerate, extracted from the roots of the desert plant Arnebia decumbens, was tested for its effect on photosynthetic electron transport system of Chlorogloeopsis fritschii. The ferricyanide-Hill reaction with water and DPC as electron donors was inhibited completely with 10-5 M shikonin isovalerate. The photoreduction of DCPIP through photosystem II was only slightly inhibited. Photosystem I from durohydroquinone to methyl viologen was not affected using 10-6 M shikonin isovalerate. The same concentration caused 49% inhibition of cyclic photophosphorylation. These results suggest that shikonin isovalerate inhibits photosynthetic electron flow at the plastoquinone pool.
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Abbreviations
- DCMU:
-
3-(3,4-dichlorophenyl)-N,N-dimethyl urea
- DBMIB:
-
2,5-dibromo-3-methyl-6-isopropyl-P-benzoquinone
- DCPIP:
-
2–6-dichlorophenolindophenol
- DPC:
-
Diphenylcarbazide
- Tricine:
-
N-[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]glycine
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Sallal, A.K.J., Nimer, N.A. Shikonin isovalerate: An inhibitor of photosystem II in Chlorogloeopsis fritschi . Arch. Microbiol. 150, 519–522 (1988). https://doi.org/10.1007/BF00408242
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DOI: https://doi.org/10.1007/BF00408242