Abstract
Intracytoplasmic membranes of the mutant strain NK3 of Rhodopseudomonas capsulata lacking the lightharvesting complex B800-850 were fused with proteoliposomes containing the B800-850 complex. Fluorescence emission spectroscopy at 77K showed that after fusion the fluorescence of the B850 bacteriochlorophyll disappeared nearly completely and the B870 fluorescence became prominent. This result and control experiments with proteoliposome-chromatophore mixture and with chromatophore and solubilized B800-850 complexes, respectively, indicate that in fused membranes a reorientation of membrane particles took place and excitons migrated from B850 to B870 bacteriochlorophyll.
In fused proteoliposome-chromatophore vesicles a light-induced carotenoid band shift was observed, reflecting the building of an electrical membrane potential due to chargeseparation. Carotenoid band shift was not observed in separated proteoliposomes and NK3 chromatophores.
It is concluded that by membrane fusion and lateral diffusion of membrane particles reaction center-light-harvesting B870 complexes came in functional contact with B800-850 antenna complexes.
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Abbreviations
- Bchl:
-
bacteriochlorophyll
- LDAO:
-
lauryl dimethylamine oxide
- RC:
-
reaction center
References
Aagaard J, Sistrom WR (1972) Control of synthesis of reaction center bacteriochlorophyll in photosynthetic bacteria. Photochem Photobiol 15:209–225
Clayton RK (1978) Physicochemical mechanisms in reaction centers of photosynthetic bacteria. In: Clayton RK, Sistrom WR (eds) The photosynthetic bacteria. Plenum Press, New York, pp 387–396
Drews G (1983) Mikrobiologisches Praktikum. Springer, Berlin Heidelberg New York, p 62
Feick R, Grondelle R van Rijgersberg CP, Drews G (1980) Fluorescence emission by wild-type and mutant strains of Rhodopseudomonas capsulata. Biochim Biophys Acta 593:241–253
Garcia AF, Drews G (1980) Characterization of three membrane fractions isolated from cells of Rhodopseudomonas capsulata adapting from chemotrophic conditions. Arch Microbiol 127:157–161
Garcia AF, Drews G (1984) Properties of membrane fractions prepared by chromatophore-liposome fusion. Z Naturforsch 39c:1112–1119
Hunter CN, van Grondelle R, Holmes NG, Jones OTG, Niederman RA (1979) Fluorescence yield properties of fraction enriched in newly synthesized bacteriochlorophyll a-protein complexes from Rhodopseudomonas sphaeroides. Photochem Photobiol 30:313–316
Kaufmann N, Reidl HH, Golecki JR, Garcia AF, Drews G (1982) Differentiation of the membrane system in cells of Rhodopseudomonas capsulata after transition from the chemotrophic to phototrophic growth conditions. Arch Microbiol 131:313–322
Kaufmann N, Hüdig H, Drews G (1984) Transposon Tn5 mutagenesis of genes of the photosynthetic apparatus in Rhodopseudomonas capsulata. Mol Gen Genet 198:153–158
Kramer HJM, Grondelle R van, Hunter CN, Westerhuis WHJ, Amesz J (1984) Pigment organization of the B800-850 antenna comples of Rhodopseudomonas sphaeroides. Biochim Biophys Acta 765:156–165
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275
Monger TG, Parson WW (1977) Singlet-triplet fusion in Rhodopseudomonas sphaeroides. Biochim Biophys Acta 460:393–407
Pearlstein RM (1982) Excitation migration and trapping in photosynthesis. Photochem Photobiol 35:835–844
Peters J, Takemoto J, Drews G (1983) Spatial relationships between the photochemical reaction center and the light-harvesting complexes in the membrane of Rhodopseudomonas capsulata. Biochemistry 22:5660–5667
Pradel J, Lavergne J, Moya I (1978) Formation and development of photosynthetic units in repigmenting Rhodopseudomonas sphaeroides wild type and “phofil” mutant strain. Biochim Biophys Acta 502:169–182
Rosenbusch JP, Garavito RM, Dorest DL, Engel A (1982) Structure and function of a pore forming transmembrane protein. Highresolution studies of a bacterial protein. In: Peeters H (ed) Protides of the biological fluids. Pergamon Press Oxford, pp 171–174
Scolnik PA, Zannoni D, Marrs BL (1980) Spectral and functional comparison between the carotenoids of the two antenna complexes of Rhodopseudomonas capsulata. Biochim Biophys Acta 593:230–240
Shiozawa JA, Welte W, Hodapp N, Drews G (1982) Studies on the size and composition of the isolated light-harvesting B800-850 pigment-protein complex of Rhodopseudomonas capsulata. Arch Biochem Biophys 213:473–485
Wraight CA, Cogdell RJ, Chance B (1978) Ion transport and electrochemical gradients in photosynthetic bacteria. In: Clayton RK, Sistrom WR (eds) The photosynthetic bacteria. Plenum Press, New York London, pp 471–511
Zankel KL (1978) Energy transfer between antenna components and raction centers. In: Clayton RK, Sistrom WR (eds) The photosynthetic bacteria. Plenum Press, New York London, pp 341–347
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Dedicated to Professor R. Clinton Fuller, Amherst, MA, USA, on the occasion of his 60th birthday in recognition of his work on photosynthetic bacteria and the cooperation between our laboratories
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Garcia, A.F., Gad'on, N. & Drews, G. Fusion of proteoliposomes containing the B800-850 light-harvesting complex with membranes of the mutant strain NK3 of Rhodopseudomonas capsulata lacking B800-850. Arch. Microbiol. 141, 239–243 (1985). https://doi.org/10.1007/BF00408065
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DOI: https://doi.org/10.1007/BF00408065