Summary
Activities of alkaline and acid phosphatases were investigated in rabbit corneas after a complete epithelial denudation in vivo (limbus to limbus). Dynamics of enzymatic changes during corneal healing were followed quantitatively in homogenates of regenerated epithelium and stroma (denuded cornea) and in cryostate frozen sections on days 1, 4, 7, 14, and 28. Biochemical and histochemical findings at these times showed a different response in each enzyme. Acid phosphatase displayed a gradual increase of activity in epithelium as well as in stroma; on day 28 after injury its content was normal. In contrast, alkaline phosphatase showed delayed activity during the repair process, and even a month after de-epithelization was still subnormal, particularly in superficial layers of epithelium.
Zusammenfassung
Untersuchung der Aktivitäten der alkalischen und sauren Phosphatasen in Kaninchenhornhäuten nach totaler Abrasio in vivo. Analyse der Dynamik der Enzymänderungen während der Wundheilungsphase. Bericht über die festgestellten biochemischen und histochemischen Beobachtungen. Die saure Phosphatase zeigte eine kontinuierliche Aktivitätszunahme im Epithel und Stroma; Am 28. Tag nach der Abrasio war die Aktivität der sauren Phosphatase normal. Dagegen wurde eine Aktivitätsverminderung der alkalischen Phosphatase während der Epithelregeneration beobachtet, sogar ein Monat nach der Abrasio fiel noch eine subnormale Aktivität, besonders in den oberflächlichen Epithelschichten auf.
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Bolková, A., Čejková, J. Biochemical and histochemical response to a complete epithelial denudation of the rabbit cornea. Albrecht von Graefes Arch. Klin. Ophthalmol. 209, 137–143 (1978). https://doi.org/10.1007/BF00407846
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DOI: https://doi.org/10.1007/BF00407846