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Selection methods in bacilli for recombinants and transformants of intra- and interspecific fused protoplasts

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Abstract

The intraspecific fusion frequencies obtained with the direct selection method on a semi-synthetic regeneration medium between strains of B. subtilis and B. licheniformis were distribution from 9.9×10-2 to 4.5×10-3, which was one or two orders higher than those of interspecific recombinations between B. subtilis and B. licheniformis.

The regeneration media were also useful for selecting interspecific transformants from plasmid carrier to non-carrier. This selection could be used as a primary selection method for inter-and intraspecific recombinants obtained by protoplast fusion.

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Abbreviations

NTG:

N-methyl-N′-nitro-N-nitrosoguanidine

Cm:

chloramphenicol

SMM:

0.5 M sucrose-0.02 M MgCl2-0.02 M maleate buffer, pH 6.5

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Authors and Affiliations

  1. Department of Applied Microbial Technology, The Kumamoto Institute of Technology, Ikeda-4-22-1, Kumamoto-city, Japan

    T. Akamatsu & J. Sekiguchi

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  1. T. Akamatsu
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  2. J. Sekiguchi
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Akamatsu, T., Sekiguchi, J. Selection methods in bacilli for recombinants and transformants of intra- and interspecific fused protoplasts. Arch. Microbiol. 134, 303–308 (1983). https://doi.org/10.1007/BF00407807

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  • DOI: https://doi.org/10.1007/BF00407807

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