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Sensitivity of yeast glycolytic enzymes to chloroquine

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Abstract

Chloroquine at pH 8.0 and 10 mM concentration inhibits about 30% glucose consumption and ethanol formation in yeast cells. Out of the 11 glycolytic enzymes assayed, phosphoglycerate kinase and pyruvate decarboxylase have been found to be most sensitive to chloroquine. Next sensitive are hexokinase, glyceraldehyde-3-phosphate dehydrogenase and pyruvate kinase. Kinetic studies with the three kinases studied revealed competitive inhibition of chloroquine with ATP (hexokinase, phosphoglycerate kinase) or ADP (pyruvate kinase).

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Author notes

  1. Henryk Kalisz

    Present address: Gesellschaft für Biotechnologische Forschung, Mascheroder Weg 1, D-3300, Braunschweig, Federal Republic of Germany

Authors and Affiliations

  1. Biochemisches Institut der Universität Freiburg, Hermann-Herder-Strasse 7, D-7800, Freiburg, Federal Republic of Germany

    Alexander Manhart, Henryk Kalisz & Helmut Holzer

  2. Geschäftsführung, Gesellschaft für Strahlen- und Umweltforschung, Ingolstädter Landstrasse 1, D-8042, Neuherberg, Federal Republic of Germany

    Helmut Holzer

Authors
  1. Alexander Manhart
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  2. Henryk Kalisz
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  3. Helmut Holzer
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Manhart, A., Kalisz, H. & Holzer, H. Sensitivity of yeast glycolytic enzymes to chloroquine. Arch. Microbiol. 150, 309–312 (1988). https://doi.org/10.1007/BF00407797

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  • DOI: https://doi.org/10.1007/BF00407797

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