Abstract
The growth of Clostridium populeti in 2% (w/v) glucose medium containing 0.2% (w/v) yeast extract was optimal with 10 mM NH4Cl as the nitrogen source. Although the maximum specific growth rate (μ=0.32 h-1) with 5 mM NH4Cl was similar, the biomass yield was about 30% lower than that at the optimum. Either sodium sulphide or cysteine-HCl at an optimum concentration of 0.33 mM and 5.0 mM respectively, could serve as the sole sulphur source for growth. The growth rate was unaffected by initial glucose concentrations of up to 10% (w/v), but in the presence of 15% glucose it declined by about 35%. The molar yield of butyric acid (mol/mol glucose) declined from 0.70 in 1% (w/v) initial glucose medium to 0.39 in 10% glucose medium. In 5.7% initial glucose medium, butyric acid levels of 6.3 g/l were obtained (0.56 mol butyrate/mol glucose) after 72 h of incubation in 2.5 l batch cultures. A decrease of about 50% in the maximum specific growth rate of C. populeti was observed in the presence of an initial concentration of either 1.2 g/l of butyric acid or 18.9 g/l of acetic acid.
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References
Andel JG van, Zoutberg GR, Crabbendam PM, Breure AM (1985) Glucose fermentation by Clostridium butyricum grown under a self generated gas atmosphere in chemostat culture. Appl Microbiol Biotechnol 23:21–26
Ballongue, J, Masion E, Amine J, Petitdemange H, Gay R (1987) Inhibitor effect of products of metabolism on growth of Clostridium acetobutylicum. Appl Microbiol Biotechnol 26:568–573
Cato EP, George WL, Finegold SM (1986) Genus Clostridium. In: Sneath PHA, Mair NS, Sharpe ME (eds) Bergey's manual of systematic bacteriology, vol 2. Wiliams and Wilkins, Baltimore, USA, pp 1141–1200
Crabbendam PM, Neijssel OM, Tempest DW (1985) Metabolic and energetic aspects of the growth of Clostridium butyricum on glucose in chemostat culture. Arch Microbiol 142:375–382
Daniels H, McCarthy JL (1948) The butyric acid fermentation of sulphite waste liquor. Paper Trade J 126:43–46
Häggström L (1986) Kinetics of product formation in batch and continuous culture of Clostridium barkeri. Appl Microbiol Biotechnol 23:187–190
Hobson PN, Reid WG, Sharma VK (1986) Production of acids by anaerobic fermentation. In: Ferranti MP, Ferrero GL, L'Hermite P (eds) Anaerobic digestion: results of research and demonstration projects. Elsevier Applied Science. New York, pp 17–21
Holdeman LV, Cato EP, Moore WEC (1977) Anaerobe laboratory manual, 4th edn, Anaerobe Laboratory. Virginia Polytechnic Institute and State University, Blacksburg, Virginia, USA, pp 146
Hsu E, Ordal ZJ (1970) Comparative metabolism of vegetative and sporulating cultures of Clostridium thermosaccharolyticum. J Bacteriol 102:369–376
Jones GA, Pickard MD (1980) Effect of titanium (III) citrate as a reducing agent on grwoth of rumen bacteria. Appl Environ Microbiol 39:1144–1147
Keith SM, MacFarlane GT, Herbert RA (1982) Dissimilatory nitrate reduction by a strain of Clostridium butyricum isolated from estuarine sediments. Arch Microbiol 132:62–66
Mountfort DO, Asher RA (1979) Effect of inorganic sulphide on the growth and metabolism of Methanosarcina barkeri strain DM. Appl Environ Microbiol 37:670–675
Patel GB (1983) Fermentation of lactose by Bacteroides polypragmatus. Can J Microbiol 29:120–128
Patel GB (1984) Ethanol production during D-xylose, L-arabinose and D-ribose fermentation by Bacteroides polypragmatus. Appl Microbiol Biotechnol 20:111–117
Patel GB, McKenzie CR (1982) Metabolism of Acetivibrio cellulolyticus during optimized growth on glucose, cellobiose and cellulose. Eur J Appl Microbiol Biotechnol 16:212–218
Patel GB, Breuil C, Agnew BJ (1982) Sulphur requirements for growth of Acetivibrio cellulolyticus. Can J Microbiol 28:772–777
Patel GB, McKenzie CR, Angew BJ (1986) Fermentation of xylose and hemicellulose hydrolysates by an ethanol-adapted culture of Bacteroides polypragmatus. Arch Microbiol 146:68–73
Playne MJ (1985) Propionic and butyric acids. In: Blanch HW, Drew S, Wang DIC (eds) Comprehensive biotechnology in industry, agriculture and medicine, vol 3, the practice of biotechnology: current commodity products. Pergamon Press, New York, pp 731–759
Schink B, Zeikus JG (1982) Microbial ecology of pectin decomposition in anoxic lake sediments. J Gen Microbiol 128:393–404
Sharpell Jr, FH (1985) Microbial flavors and fragrances. In: Blanch HW, Drew S, Wang DIC (eds) Comprehensive biotechnology in industry, agriculture and medicine, vol 3. The practice of biotechnology: current commodity products. Pergamon Press, New York, pp 965–981
Sharpell Jr, FH, Stegmann C (1981) Development of fermentation media for the production of butyric acid. In: Moo-Young M, Robinson CW (eds) Advances in biotechnology, vol 2, Pergamon Press, Toronto, Canada, pp 71–77
Sleat R, Mah RA (1985) Clostridium populeti sp. nov., a cellulolytic species from a woody-biomass digestor. Int J Syst Bacteriol 35:160–163
Wolin EA, Wolin MJ, Wolfe RS (1963) Formation of methane by bacterial extracts. J Biol Chem 238:2882–2886
Zehnder AJB, Wuhrmann K (1976) Titanium (III) citrate as a nontoxic oxidation-reduction buffering system for the culture of obligate anaerobes. Science 194:1165–1166
Zeikus JG (1980) Chemical and fuel production by anaerobic bacteria. Ann Rev Microbiol 34:423–464
Zeikus JG, Lynd LH, Thompson TE, Krzycki JA, Weimer PJ, Hegge PW (1980) Isolation and characterization of a new, methylotrophic, acidogenic anaerobe, the Marburg strain. Curr Microbiol 3:381–386
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Patel, G.B., Agnew, B.J. Growth and butyric acid production by Clostridium populeti . Arch. Microbiol. 150, 267–271 (1988). https://doi.org/10.1007/BF00407790
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DOI: https://doi.org/10.1007/BF00407790