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Effect of Prostaglandin E2 on aqueous humor flow in the rabbit eye

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Summary

The examinations were carried out in albino rabbits under urethane anaestesia. After intracameral injection of 0.5 μg Prostaglandin E2, dissolved in 10 μl 10% ethanol, intraocular pressure increased by 20.5±4.9 mm Hg. Aqueous humor protein concentration in the uninjected control eyes was 0.176±0.03 g%. Thirty minutes after intracameral injection of 10 μl 10% ethanol solution the concentration rose to 0.38±0.19 g-%, and after of 0.5 μg Prostaglandin E2 to 1.85 ±0.41 g% These interventions were not followed by significant changes in aqueous humor osmolarity (normal value 313.7±17.9 mOsm, after ethanol 330 5±18. 0, and after prostaglandin E2 329.3±6.9).

In a special series of experiments the rate of aqueous humor production was determined. In the uninjected control eyes a value of 1.57±0.61 μl/min was found, but after intracameral injection of 0.5 μg prostaglandin E2, 5.45±1.99 μl/min.

The authors draw the conclusion that prostaglandin E2 increases intraocular tension not by enhancing aqueous humor production but by disrupting the bloodaqueous humor barrier.

Zusammenfassung

Die Untersuchungen wurden an Albinokaninchen in Urethannarkose durchgeführt. Nach Injektion von 0,5 μg Prostaglandin E2 (in 10 μl10%iger Äthanollösung) in die Vorderkammer stieg der Augenbinnendruck um 20,5±-4,9 mm Hg an. Der Gesamteiweißgehalt im Kammerwasser der intakten Kontrollaugen betrug 0,176±0,03 g-%. 30 min nach Einspritzung von 10 μl 10%iger Äthanollösung in die Vorderkammer erhöhte sich der Gesamteiweißgehalt auf 0,38±0,19 g-%. Diese Einwirkungen hatten keine signifikante Änderung der Kammerwasser-Osmolarität zur Folge (Normalwert 313,7±17,9 mOsm, nach Äthanol 330,5±18,1 und nach Prostaglandin E2 329,3±6,9).

In einer besonderen Versuchsreihe wurde die Kammerwasserproduktion bestimmt. In den intakten Kontrollaugen ergab sich hierbei ein Wert von 1,57±0,61 (μl/m, nach Gabe von 0,5 μg Prostaglandin E2 aber 5,45±1,99 μl/min.

Die Autoren ziehen den Schluß, daß Prostaglandin E2 den Augenbinnendruck nicht durch Steigerung der Kammerwasserproduktion, sondern durch Schädigung der Blut-Kammerwasserschranke erhöht.

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Authors and Affiliations

  1. University Bye Clinic and Central Clinical Laboratory, Pécs, Hungary

    Stephen Takáts, Casimir Jobst & Hilda Szilvássy

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  1. Stephen Takáts
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  2. Casimir Jobst
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  3. Hilda Szilvássy
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Takáts, S., Jobst, C. & Szilvássy, H. Effect of Prostaglandin E2 on aqueous humor flow in the rabbit eye. Albrecht v. Graefes Arch. klin. exp. Ophthal. 190, 221–227 (1974). https://doi.org/10.1007/BF00407096

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