Skip to main content
SpringerLink
Log in

Experimental separation of cell division and silica shell formation in Cyclotella cryptica

  • Published:
Archiv für Mikrobiologie Aims and scope Submit manuscript

Summary

  1. 1.

    Cytokinesis is inhibited in Cyclotella cryptica, when the cells are subjected to Ca++ or Mg++ deficiency. As a result of such inhibition, two lateral silica shells are formed. These shells surround the cell cylinder of the diatom at the median region between the intercalary connecting bands.

  2. 2.

    Lateral silica shells are distinguished from the normal valves by being slightly rolled and having their marginal region flattened. Both kinds of shells possess the same type of fine structures.

  3. 3.

    Mitotic inhibition through colchicine induces more than one pair of lateral silica shells which appear on the undivided giant cells showing plasmatic growth. Similar giant cells with many lateral shells are produced in the presence of β-phenethyl alcohol.

  4. 4.

    It was concluded that neither the achievement of cytokinesis nor mitosis are prerequistes for lateral silica shell formation. It is rather dependent upon plasmatic growth.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Badour, S. S. A.: Kennzeichnung von Mineralsalzmangelzuständen bei Grünalgen mit analytisch-chemischer Methodik. II. Phosphatfraktionen bei Kaliummangel im Vergleich mit Magnesium- und Manganmangel. Flora (Jena) 151, 99–119 (1961).

    Google Scholar 

  • Berrah, Ghoulem, and Walter A. Konetzka: Selective and reversible inhibition of the synthesis of bacterial deoxyribonucleic acid by phenethyl alcohol. J. Bact. 83, 738–744 (1962).

    PubMed  Google Scholar 

  • Ceriotti, G.: A new technique for the determination of DNA. J. biol. Chem. 198, 297–299 (1952).

    PubMed  Google Scholar 

  • —: The use of orcinol-test. J. biol. Chem. 214, 59–68 (1955).

    PubMed  Google Scholar 

  • Coombs, J., P. J. Halicki, O. Holm-Hansen, and B. E. Volcani: Studies on the biochemistry and fine structure of silica shell formation in diatoms. I. Changes in concentration of nucleoside triphosphates during synchronized division of Cylindrotheca fusiformis Reimann and Lewin. Exp. Cell Res. 47, 302–314 (1967).

    PubMed  Google Scholar 

  • —: Studies on the biochemistry and fine structure of silica shell formation in diatoms. II. Changes in concentration of nucleoside triphosphates in siliconstarvation synchrony of Navicula pelliculosa (Bréb.) Hilse. Exp. Cell Res. 47, 315–328 (1967).

    PubMed  Google Scholar 

  • Engel, W., u. L. Holzapfel: Kieselsäurenachweis in Gegenwart von Phosphorsäure und Eiweißkomponenten in biologischen Flüssigkeiten: Analytische Nachweis- und Trennungsmethoden. Beitr. Silikose-Forsch. 4, 67–71 (1960).

    Google Scholar 

  • Frank, E.: Vergleichende Untersuchungen zum Calcium-, Kalium-und Phosphathaushalt von Grünalgen. II. Calciummangel bei Hydrodictyon, Sphaeroplea und Chlorella. Flora (Jena) 152, 157–167 (1962).

    Google Scholar 

  • Galling, G.: Analyse des Magnesium-Mangels bei synchronisierten Chlorellen. Arch. Mikrobiol. 46, 150–184 (1963).

    PubMed  Google Scholar 

  • Geitler, L.: Das Auftreten zweier obligater, metagamer Mitosen ohne Zellteilung während der Bildung der Erstlingsschalen bei den Diatomeen. Ber. dtsch. bot. Ges. 66, 222–227 (1953a).

    Google Scholar 

  • —: Abhängigkeit der Membranbildung von der Zellteilung bei Diatomeen und differentielle Teilungen im Zusammenhang mit der Bildung der Innenschalen. Planta (Berl.) 43, 75–82 (1953b).

    Google Scholar 

  • Henee, K., and W. W. N. Warren: Cell morphology of a human diploid cell strain (WI-38) after treatment with arabinosylcytosine. Cancer Res. 27, 242–250 (1967).

    PubMed  Google Scholar 

  • Kern, H.: Zur quantitativen Bestimmung von Nucleinsäuren. Planta (Berl.) 55, 259–273 (1960).

    Google Scholar 

  • Knutsen, G.: Effects of phenylethyl alcohol on Chlorella pyrenoidosa. Physiol. Plant. 19, 142–151 (1966).

    Google Scholar 

  • Kuhl, A., and H. Lorenzen: Handling and culturing of Chlorella. In: Methods in cell physiology. (Ed. D. M. Presscott), Vol. I, pp. 159–187. New York: Academic Press 1964.

    Google Scholar 

  • Lewin, J. C.: Silicon metabolism in diatoms. IV. Growth and frustule formation in Navicula pelliculosa. Canad. J. Microbiol. 3, 427–433 (1957).

    Google Scholar 

  • —: Silicification. In: Physiology and Biochemistry of Algae. (Ed. R. A. Lewin), pp. 445–455. New York: Academic Press 1962.

    Google Scholar 

  • Mechsner, K.: Untersuchungen an Chlorella vulgaris über den Einfluß der Alkali-Ionen auf die Lichtphosphorylierung. Biochim. biophys. Acta (Amst.) 33, 150–158 (1959).

    Article  Google Scholar 

  • O'Kelley, J. C., and W. R. Herndon: Effect of strontium replacement for calcium on production of motile cells in Protosiphon. Science 130, 718 (1959).

    PubMed  Google Scholar 

  • —: Alkaline earth elements and zoospore release and development in Protosiphon botryoides. Amer. J. Bot. 48, 796–802 (1961).

    Google Scholar 

  • Reimann, B. E. F.: Deposition of silica inside a diatom cell. Exp. Cell Res. 34, 605–608 (1964).

    PubMed  Google Scholar 

  • —, J. C. Lewin, and R. R. L. Guillard: Cyclotella cryptica, a new brackish-water diatom species. Phycologia 3, 75–84 (1963).

    Google Scholar 

  • — and B. E. Volcani: Studies on the biochemistry and fine structure of silica shell formation in diatoms. I. The structure of the cell wall of Cylindrotheca fusiformis Reimann and Lewin. J. Cell Biol. 24, 39–55 (1965).

    Article  PubMed  Google Scholar 

  • Roe, J. H.: The determination of sugar in blood and spinal fluid with anthrone reagent. J. biol. Chem. 212, 335–343 (1955).

    PubMed  Google Scholar 

  • Ruppel, H. G.: Untersuchungen über die Zusammensetzung von Chlorella bei Synchronisation im Licht-Dunkel-Wechsel. Flora (Jena) 152, 113–138 (1962).

    Google Scholar 

  • Salama, A. M., and M. I. Naguib: Colchicine after effects on the absorption and utilization of sucrose by Cunninghamella sp. Arch. Mikrobiol. 48, 185–202 (1964).

    Google Scholar 

  • Schlösser, U.: Enzymatisch gesteuerte Freisetzung von Zoosporen bei Chlamydomonas reinhardii Dangeard in Synchronkultur. Arch. Mikrobiol. 54, 129–159 (1966).

    Google Scholar 

  • Stoermer, E. F., H. S. Pankratz, and C. C. Bowen: Fine structure of the diatom Amphipleura pellucida. II. Cytoplasmic fine structure and frustule formation. Amer. J. Bot. 52, 1067–1078 (1965).

    Google Scholar 

  • Stosch, H. A., v.: Über das Perizonium der Diatomeen. Vortr. Gesamtgebiet Bot., hrsg. Dtsch. Bot. Ges. N. F. 1, 43–52 (1962).

    Google Scholar 

  • Wanka, F.: Die Bestimmung der Nucleinsäuren in Chlorella-Kulturen. Planta (Berl.) 58, 594–609 (1962).

    Google Scholar 

  • Werner, D.: Die Kieselsäure im Stoffwechsel von Thalassiosira fluviatilis Hust. Diss., Göttingen 1965.

  • —: Die Kieselsäure im Stoffwechsel von Cyclotella cryptica. Arch. Mikrobiol. 55, 278–308 (1966).

    Google Scholar 

  • Zahn, R. K., E. Tiesler, B. Heicke, W. Hanske, W. Forster, H. Hollstein, and H. Walter: Cellular division and cellular volume distribution in the presence of 2-phenylethanol and some of its derivatives. Nature (Lond.) 212, 298 (1966).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Plant Physiology, University of Göttingen, Germany

    S. S. Badour

Authors
  1. S. S. Badour
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Badour, S.S. Experimental separation of cell division and silica shell formation in Cyclotella cryptica . Archiv. Mikrobiol. 62, 17–33 (1968). https://doi.org/10.1007/BF00407050

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00407050

Keywords

Search

Navigation