Summary
The microsomal, mitochondrial, and nuclear fractions of cutaneous tumors have been investigated. Cutaneous tumors were homogenized and microsomal, mitochondrial, and nuclear fractions were purified. The membrane proteins were solubilized with sodium duodecyl sulfate (SDS). The membrane lipids were removed and membrane proteins were solubilized again in a small volume of SDS solution and chromatographed in SDS-acrylamide slab-gel. The plates were stained with Coomassie Brilliant Blue to show protein bands. The preliminary results show that the electrophoretic profiles of microsomal proteins are characteristic of some tumors.
Zusammenfassung
Die mikrosomale, mitochondriale und nukleare Fraktion cutaner Tumoren wurde untersucht. Dazu wurden diese cutanen Tumoren homogenisiert und die mikrosomale, mitochondriale und nukleare Fraktion gereinigt. Die Membranproteine wurden in Natriumduodecylsulfat (SDS) gelöst. Die Membranlipide wurden entfernt und die Membranproteine wiederum in einem kleinen Volumen von SDS gelöst und in SDS-Acrylamid-Slab-Gel chromatographiert. Die Proteinbande wurden mit Coomassie Brilliant-blau gekennzeichnet. Die vorläufigen Resultate zeigen, daß das elektrophoretische Profil der mikrosomalen Proteine für manche Tumoren charakteristisch ist.
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Supported by a grant from the Foundation for Research in Dermatology
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Landi, F.L., Cardillo, A., Fabrizi, G. et al. Membrane proteins in human skin tumors. Arch Dermatol Res 271, 419–427 (1981). https://doi.org/10.1007/BF00406687
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DOI: https://doi.org/10.1007/BF00406687