Summary
Experimental cutaneous Candida albicans infections in guinea pigs are histologically characterized by intense epidermal polymorphonuclear leukocyte (PMN) accumulation. To study the role of PMNs in vivo, we injected 250 μcolchicine/kg i.p., a strong inhibitor of PMN chemotaxis, and observed the influence of reduced PMN migration in experimental cutaneous candidiasis with special interest in the elimination course of the organisms.
There was a significant delay in the clearance of the organisms in the colchicine-treated group with decreased epidermal PMN infiltration and prolonged visible scaling process.
Our data suggest that the delay of epidermal PMN migration parallels the disappearance of the organisms from the infected skin and that PMNs play an important part in defense against candida infections especially in the elimination process.
Zusammenfassung
Experimentelle Hautinfektion mit Candida albicans bei Meerschweinchen wird histologisch durch intensive epidermische Akkumulation der polymorphonuclearen Leukocyten (PMN) charakterisiert. Um die Rollen der PMN in vivo zu studieren, injizierten wir i.p. 250 μg Colchicin/kg, einen potenten Inhibitor der PMN-Chemotaxis, und beobachteten Einflüsse der reduzierten PMN-Migration bei der experimentellen Candidiasis, mit spezialem Interesse am Eliminationsverlauf des Organismus.
Es gab bei der Colchicin-behandelten Gruppe eine signifikante Verzögerung mit der Abnahme der epidermischen PMN-Infiltration und der Verlängerung des sichtbaren Schuppenprozesses.
Unsere Daten zeigen, daß die Verzögerung der epidermischen PMN-Migration dem Verschwinden des Organismus aus der infizierten Haut entspricht und daß PMN in der Abwehr gegen die Candida-Infektion, besonders in dem Eliminationsverlauf, eine wichtige Rolle spielen.
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Miyachi, Y., Horio, T. & Imamura, S. The fate of experimental cutaneous candidiasis in guinea pigs under the suppressed polymorphonuclear leukocyte chemotaxis by colchicine. Arch Dermatol Res 271, 373–380 (1981). https://doi.org/10.1007/BF00406681
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DOI: https://doi.org/10.1007/BF00406681