Summary
Two tumor cell-aggregation factors of glycoprotein nature, separated from rat ascites hepatoma AH136B cells (forming cell islands in vivo), had different antigenicity; one was not absorbed by immunoadsorbent chromatography with anti-rat serum antibody and the other was. The unabsorbed factor induced aggregation (as shown in the form of simple apposition) of rat ascites hepatoma AH109A cells (present as a free form in vivo) and cell adhesiveness characterized by well-defined tripartite junctional complexes, including intermediate junctions, desmosomes, and tight junctions. In contrast, the absorbed factor from AH136B cells, AH109A cells or normal rat serum aggregated AH109A cells but failed to develop the junctional complexes; only simple apposition was observed. AH109A cells themselves contained the absorbed factor but not the unabsorbed factor. AH136B cells proliferating in the skin developed the junctional complexes, while AH109A cells proliferating in the skin did not from any junctional complexes.
Similar content being viewed by others
References
Anservin, K. D.: The effect of RNA synthesis inhibition and of protein synthesis inhibition on embryonic induction and cell differentiation in rana pipiens. J. Morphol. 117, 171–184 (1965)
Farquhar, M. G., Palade, G. E.: Junctional complexes in various epithelia. J. Cell Biol. 17, 375–412 (1963)
Gasic, G. J., Galanti, N. L.: Proteins and disulfide groups in the aggregation of dissociated cells of sea sponges. Science, 151, 203–205 (1966)
Giraud, A., Fayet, G., Lissitzky, S.: Thyrotropin-induced aggregation-promoting factors of adult cultured thyroid cells. Expl. Cell Res. 87, 359–364 (1974)
Hanaoka, Y., Kudo, K., Ishimaru, Y., Hayashi, H.: Biochemical comparison of two tumour cell aggregation factors from rat ascites hepatoma cells. Br. J. Cancer 31, 536–544 (1978)
Ishihara, H., Ishimaru, Y., Hayashi, H.: Ultrastructural changes of intercellular junctions in rat ascites hepatoma cells with calcium depletion. Br. J. Cancer 35, 643–656 (1977)
Ishimaru, Y., Ishihara, H., Hayashi, H.: An electron microscopic studies of tumour cell adhesiveness induced by aggregation promoting factor from rat ascites hepatoma cells. Br. J. Cancer 31, 207–217 (1975)
Ishimaru, Y., Kudo, K., Ishihara, H., Hayashi, H.: The induction of tumour cell adhesiveness and intercellular junction by a glycoprotein of rat ascites hepatoma cell surface. Br. J. Cancer 34, 426–436 (1976)
Kudo, K., Hanaoka, Y., Hayashi, H.: Characterization of tumour cell aggregation promoting factor from rat ascites hepatoma cells: Separation of two factors with different antigenic propaty. Br. J. Cancer 33, 79–90 (1976a)
Kudo, K., Hanaoka, Y., Hayashi, H.: Purification of tumour cell aggregation promoting factor associated with rat ascites hepatoma cell surface. Br. J. Cancer 34, 88–89 (1976b)
Kudo, K., Tasaki, I., Hanaoka, Y., Hayashi, H.: A tumour cell aggregation promoting substance from rat ascites hepatoma cells. Br. J. Cancer, 30, 549–559 (1974)
lentz, T., Trinkaus, J. P.: Differentiation of the junctional complex of surface cells in the developing fundulus blastoderm. J. Cell Biol. 48, 455–472 (1971)
Lowry, O. H., Rosebrough, N., Fan, A. L., Randall, R. L.: Protein measurement with the folin phenol reagent. J. Biol. Chem. 193, 265–275 (1951)
Müller, W. E. G., Müller, I., Kurelec, B., Zahn, R. K.: Species-specific aggregation factor in sponges. IV. Inactivation of the aggregation factor by mucoid cells from another species. Expl. Cell Res., 98, 31–40 (1976)
Odashima, S.: Comparative studies on the transplantability of liver cancers induced in rat fed with 3-methyl-4-dimethylaminoazobenzene for 3–6 months. Gann 53, 325–348 (1962)
Odashima, S.: Establishment of ascites hepatoma in the rat. J. natn. Cancer Inst. Monog. 16, 51–71 (1964)
Reich, E., Franklin, R. M., Shatkin, A. J., Tatum, E. L.: Effect of actinomycin D on cellular nucleic acid synthesis and virus production. Science, N.Y., 134, 556–557 (1961)
Trelstad, R. L., Hay, E. D., Revel, J. P.: Cell contact during early morphogenesis in the chick embryo. Develop. Biol. 16, 78–106 (1967)
Yoshida, T., Isaka, H., Hanamura, H., Odashima, S., Satoh, H.: Studies on rat ascites hepatoma cells. Trans. Soc. Path. Jap. 44, 407–421 (in Japanese) (1955)
Yoshida, H., Ozaki, T., Ushijima, K., Hayashi, H.: Studies on the mechanisms of invasion in cancer. I. Isolation and purification of a factor chemotactic for cancer cells. Int. J. Cancer, 6, 123–132 (1970)
Author information
Authors and Affiliations
Additional information
This is No. 7 of the studies on tumor cell aggregation-promoting factor
Rights and permissions
About this article
Cite this article
Ishimaru, Y., Kudo, K., Koga, Y. et al. A possible mechanism for island formation by rat ascites hepatoma cells with special reference to the function of aggregation factor at the cell surface. J Cancer Res Clin Oncol 93, 123–136 (1979). https://doi.org/10.1007/BF00406570
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00406570