Abstract
Induction of synthesis of cellulolytic enzymes in Trichoderma reesei QM 9414 by cellobiono-1,5-lactone (CBL) has been investigated in a replacement system lacking additional carbon source. CBL induced cellulase secretion optimally at pH 5 and a concentration of 70 μg/ml. Higher concentrations lead to lower induction. De novo induction of cellulases was proven by the inhibitory effect of cycloheximide addition. Induction by CBL was shown to act synergistically on induction by sophorose, as it decreased the concentration of sophorose required for maximal induction. Maximal endo-β-1,4-glucanase activities induced by either sophorose or CBL were comparable. The CBL-induced cellulase system contained all the major cellulolytic enzymes of T. reesei, i.e. cellobiohydrolase I and II, and endoglucanase I, as shown by SDS-PAGE, Western blotting and detection with specific mono- and polyclonal antibodies. No differences were seen in the types of individual enzymes formed upon induction by either sophorose or CBL. No other hydrolytic enzymes appear to be induced by CBL (i.e. amylase, laminarinase, xylanase).
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- SDS-PAGE:
-
polyacrylamide gel electrophoresis in the presence of sodium-dodecylsulfate
- CBL:
-
cellobiono-1,5-lacton
- CBH:
-
cellobiohydrolase
- EG:
-
endoglucanase
- IgG:
-
immunoglobulin G
References
Ayers A, Ayers SB, Eriksson KE (1978) Cellobiose oxidase, purification and partial characterization of a hemoprotein from Sporotrichum pulverulentum. Eur J Biochem 90:171–181
Bruchmann EE, Graf H, Saad AA, Schrenk D (1978) Zur Gewinnung hochaktiver Cellulasepräparate und Optimierung der enzymatischen Cellulosehydrolyse II. Chem Ztg 102:154–155
Bruchmann EE, Schach H, Graf H (1987) Role and properties of lactonase in a cellulase system. Biotechnol Appl Biochem 9:146–159
Bruchmann EE, Seethaler D, Schroth T, Graf H (1988) Zur Gewinnung hochaktiver Cellulasepräparate und Optimierung der enzymatischen Cellulosehydrolyse III. Chem Ztg 112:7–8
Enari TM, Niku-Paavola ML (1987) Enzymatic cellulose hydrolysis. CRC Crit Rev Biotechnol 5:67–87
Frush HL, Isbell HS (1963) Aldonic acids, lactonization of aldonic acids. Meth Carbohyd Chem 2:14–18
Glenn M, Ghosh A, Ghosh BK (1985) Subcellular fractionation of a hypercellulolytic mutant of Trichoderma reesei Rut C-30: localization of endoglucanase in microsomal fraction. Appl Environ Microbiol 50:1137–1143
Hrmova M, Biely P, Vrsanska M (1986) Specificity of cellulase and β-xylanase induction in Trichoderma reesei QM 9414. Arch Microbiol 144:307–311
Knowles J, Lehtovaara P, Teeri TT (1987) Cellulase families and their genes. Trends Biotechnol 5:255–261
Kubicek CP (1987) Involvement of a conidial endoglucanase and a plasma-membrane bound β-glucosidase in the induction of endoglucanase synthesis by cellulose in Trichoderma reesei. J Gen Microbiol 133:1481–1487
Kubicek CP, Panda T, Schreferl-Kunar G, Gruber F, Messner R (1987) O-linked but not N-linked glycosylation is necessary for the secretion of endoglucanases I and II by Trichoderma reesei. Can J Microbiol 33:698–703
Kubicek CP, Mühlbauer G, Klotz M, John E, Kubicek-Pranz EM (1988) Properties of a conidial-bound cellulase enzyme system from Trichoderma reesei. J Gen Microbiol 134:1215–1222
Labudova I, Farkas V (1983) Multiple forms in the enzyme system of Trichoderma reesei during its growth on cellulose. Biochim Biophys Acta 744:135–140
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (Lond) 227:680–685
Lovrien RE, Gusek T, Hart B (1985) Cellulase and protease specific activities of commercially available cellulase preparations. J Appl Biochem 7:258–272
Mandels M, Andreotti RE (1978) Problems and challenges in the cellulose to cellulase fermentation. Proc Biochem 13:6–13
Mandels M, Parrish FW, Reese ET (1962) Sophorose as an inducer of cellulase in Trichoderma viride. J Bacteriol 83:400–408
Merivuori H, Siegler KM, Sands JA, Montenecourt BS (1984) Regulation of cellulase biosynthesis and secretion in fungi. Biochem Soc Trans 13:411–414
Messner R, Kubicek CP (1988) Intracellular precursers of endo-β-1,4-glucanase in Trichoderma reesei. FEMS Microbiol Lett 50:227–232
Messner R, Gruber F, Kubicek CP (1988) Differential regulation of synthesis of multiple forms of specific endoglucanases by Trichoderma reesei QM 9414. J Bacteriol 170:3689–3693
Mischak H, Hofer F, Messner R, Weissinger E, Hayn M, Tomme P, Esterbauer H, Küchler E, Claeyssens M, Kubicek CP (1989) Monoclonal antibodies against different domains of cellobiohydrolase I and II from Trichoderma reesei. Biochim Biophys Acta 990:1–7
Sternberg D, Mandels GR (1979) Induction of cellulolytic enzymes in Trichoderma reesei by sophorose. J Bacteriol 139:761–769
Sternberg D, Mandels GR (1982) β-glucosidase induction and repression in the cellulolytic fungus Trichoderma reesei. Exp Mycol 6:115–124
Umile C, Kubicek CP (1986) A constitutive, plasma-membrane bound β-glucosidase in Trichoderma reesei. FEMS Microbiol Lett 34:291–295
Vaheri MP (1982) Acidic degradation products of cellulose during enzymatic hydrolysis by Trichoderma reesei. J Appl Biochem 4:153–160
Vaheri MP (1983) Formation of oxidative activity for the initial degradation of crystalline cellulose by Trichoderma reesei. J Appl Biochem 5:66–74
Vaheri MP, Leisola M, Kaupinnen V (1979) Transglycosylation products of the cellulase system of Trichoderma reesei. Biotechnol Letts 1:41–46
Westermark U, Erikson KE (1974) Cellobiose-quinone oxidoreductase, a new wood degradating enzyme from white rot fungi. Acta Chem Scand B 28:209–214
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Iyayi, C.B., Bruchmann, E.E. & Kubicek, C.P. Induction of cellulase formation in Trichoderma reesei by cellobiono-1,5-lacton. Arch. Microbiol. 151, 326–330 (1989). https://doi.org/10.1007/BF00406559
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00406559