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Inhibitory effect of 8-methoxysporalen plus UVA (PUVA) on the systemic induction of contact sensitivity to dinitrochlorbenzene (DNCB) in guinea pigs

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Summary

To investigate the influence of 8-MOP and UVA on the induction of cell-mediated immunity, guinea pigs, sensitized with a single injection of dinitrofluorbenzene (DNFB) in Freund's complete adjuvant (FCA) in all footpads and the nuchal skin, were treated with 8-MOP, UVA, or 8-MOP+ UVA on days 0, 2, and 5 and tested with dinitrochlorbenzene (DNCB) on day 14 after sensitization. Two control groups, exposed in a covered condition to the PUVA 4000 lamp, to observe the heat effect, showed a slightly enhanced contact sensitivity in comparison to the only sensitized control group. No altered reactivity was observed after irradiation with longwave UV light alone, whereas a statistically significant enhanced resp. reduced contact sensitivity was obtained after the treatment with 8-MOP alone and the combined action of 8-MOP +UVA, respectively.

Zusammenfassung

Um etwas über den Einfluß der PUVA-Behandlung auf die Induktion der cellulären Immunität zu erfahren, wurden Meerschweinchen, die durch eine einmalige Injektion von Dinitrofluorbenzol (DNFB) in Freund's komplettem Adjuvans (FCA) in die Pfoten und die Nackenhaut sensibilisiert worden waren, an den Tagen 0, 2 und 5 mit 8-MOP, UVA oder 8-MOP+UVA behandelt und 14 Tage nach der Sensibilisierung mit Dinitrochlorbenzol (DNCB) getestet. Zwei Kontrollgruppen, behandelt mit oder ohne 8-MOP wurden, zugedeckt mit chirurgischen Tüchern, unter die PUVA 4000 Lampe gelegt, um den Wärmeeffekt zu prüfen. Beide Gruppen zeigten eine leicht erhöhte Kontaktüberempfindlichkeit. Keine veränderte Reaktivität wurde nach UVA allein beobachtet, während sich eine statistisch signifikant erhöhte, bzw. erniedrigte Kontaktüberempfindlichkeit nach 8-MOP allein, bzw. 8-MOP +UVA ergab.

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Authors and Affiliations

  1. Department of Dermatology, University Hospital Zürich (Director: Prof. Dr. U. W. Schnyder), CH-8091, Zürich, Switzerland

    J. Ch. Meyer, H.-P. Grundmann & H. Weiss

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  1. J. Ch. Meyer
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  2. H.-P. Grundmann
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  3. H. Weiss
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Dedicated to Prof. Dr. P. Laugier, Chairman of the Department of Dermatology, University of Geneva, Switzerland, on the occasion of his 70th anniversary

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Meyer, J.C., Grundmann, HP. & Weiss, H. Inhibitory effect of 8-methoxysporalen plus UVA (PUVA) on the systemic induction of contact sensitivity to dinitrochlorbenzene (DNCB) in guinea pigs. Arch Dermatol Res 269, 189–195 (1980). https://doi.org/10.1007/BF00406539

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  • DOI: https://doi.org/10.1007/BF00406539

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