Abstract
The rubredoxin content of Acinetobacter calcoaceticus in dependence on the carbon source (acetate, n-alkanes, succinate, L-malate) and on the growth phase was studied by means of a radioimmunoassay. The method used was specific for rubredoxin from Acinetobacter calcoaceticus. The formation of rubredoxin increased with time up to the end of the logarithmic phase when n-alkanes were the sole carbon source. After growth of Acinetobacter calcoaceticus on non-hydrocarbon substrates, rubredoxin was not detected.
References
Asperger, O., Kleber, H.-P., Aurich, H.: Zytochrom-Zusammensetzung von Acinetobacter calcoaceticus. Acta biol. Med. Germ. 37, 191–198 (1978)
Aurich, H., Sorger, D., Asperger, O.: Isolierung und Charakterisierung eines Rubredoxins aus Acinetobacter calcoaceticus. Acta biol. Med. Germ. 35, 443–451 (1976)
Bruschi, M., Hatchikian, C. E., Golovleva, L. A., Le Gall, J.: Purification and characterization of cytochrome c3, ferredoxin, and rubredoxin isolated from Desulfovibrio desulfuricans Norway. J. Bacteriol. 129, 30–38 (1977)
Cardini, G., Jurtshuk, P.: The enzymatic hydroxylation of n-octane by Corynebacterium sp. strain 7E1C. J. Biol. Chem. 245, 2789–2796 (1970)
Claus, R., Asperger, O., Kleber, H.-P.: Nachweis und partielle Anreicherung von Rubredoxin-Reductase aus Acinetobacter calcoaceticus. Wiss. Z. Karl-Marx-Univ. Leipzig, Math.-Nat. R. 27, 17–24 (1978)
Claus, R., Hädge, D., Asperger, O., Fiebig, H., Kleber, H.-P.: Quantitative immunologische Bestimmungsmethode für Rubredoxin in Bakterienrohextrakten von Acinetobacter calcoaceticus. Z. Allg. Mikrobiol. 20, 95–103 (1980)
Kleber, H.-P., Aurich, H.: Einfluß von n-Alkanen auf die Synthese der Enzyme des Glyoxylatzyklus in Acinetobacter calcoaceticus. Z. Allg. Mikrobiol. 13, 473–480 (1973)
Kleber, H.-P., Schöpp, W., Aurich, H.: Verwertung von n-Alkanen durch einen Stamm von Acinetobacter calcoaceticus. Z. Allg. Mikrobiol. 13, 445–447 (1973)
Le Gall, J., Dragoni, N.: Dependence of sulphite reduction on a crystallized ferredoxin from Desulfovibrio gigas. Biochem. Biophys. Res. Commun. 23, 145–149 (1966)
Lovenberg, W., Sobel, B.: Rubredoxin — a new electron transfer protein from Cl. pasteurianum. Proc. Natl. Acad. Sci. USA 54, 193–199 (1965)
Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J.: Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265–275 (1951)
Meyer, T. E., Sharp, J. J., Bartsch, R. G.: Isolation and properties of rubredoxin from the photosynthetic green sulfur bacteria. Biochim. Biophys. Acta 234, 266–269 (1971)
Newman, D. J., Postgate, J. R.: Rubredoxin from a nitrogen-fixing variety of Desulfovibrio desulfuricans. Eur. J. Biochem. 7, 45–50 (1968)
Peterson, J. A.: Cytochrome content of two Pseudomonads containing mixed-function oxidase systems. J. Bacteriol. 103, 714–721 (1970)
Peterson, J. A., Basu, D., Coon, M. J.: Enzymatic ω-oxidation. I. Electron carriers in fatty acid and hydrocarbon hydroxylation. J. Biol. Chem. 241, 5162–5164 (1966)
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Claus, R., Asperger, O. & Kleber, HP. Influence of growth phase and carbon source on the content of rubredoxin in Acinetobacter calcoaceticus . Arch. Microbiol. 128, 263–265 (1980). https://doi.org/10.1007/BF00406170
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DOI: https://doi.org/10.1007/BF00406170