Skip to main content
Log in

Influence of growth phase and carbon source on the content of rubredoxin in Acinetobacter calcoaceticus

  • Short Communication
  • Published:
Archives of Microbiology Aims and scope Submit manuscript

Abstract

The rubredoxin content of Acinetobacter calcoaceticus in dependence on the carbon source (acetate, n-alkanes, succinate, L-malate) and on the growth phase was studied by means of a radioimmunoassay. The method used was specific for rubredoxin from Acinetobacter calcoaceticus. The formation of rubredoxin increased with time up to the end of the logarithmic phase when n-alkanes were the sole carbon source. After growth of Acinetobacter calcoaceticus on non-hydrocarbon substrates, rubredoxin was not detected.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

References

  • Asperger, O., Kleber, H.-P., Aurich, H.: Zytochrom-Zusammensetzung von Acinetobacter calcoaceticus. Acta biol. Med. Germ. 37, 191–198 (1978)

    PubMed  Google Scholar 

  • Aurich, H., Sorger, D., Asperger, O.: Isolierung und Charakterisierung eines Rubredoxins aus Acinetobacter calcoaceticus. Acta biol. Med. Germ. 35, 443–451 (1976)

    PubMed  Google Scholar 

  • Bruschi, M., Hatchikian, C. E., Golovleva, L. A., Le Gall, J.: Purification and characterization of cytochrome c3, ferredoxin, and rubredoxin isolated from Desulfovibrio desulfuricans Norway. J. Bacteriol. 129, 30–38 (1977)

    PubMed  Google Scholar 

  • Cardini, G., Jurtshuk, P.: The enzymatic hydroxylation of n-octane by Corynebacterium sp. strain 7E1C. J. Biol. Chem. 245, 2789–2796 (1970)

    PubMed  Google Scholar 

  • Claus, R., Asperger, O., Kleber, H.-P.: Nachweis und partielle Anreicherung von Rubredoxin-Reductase aus Acinetobacter calcoaceticus. Wiss. Z. Karl-Marx-Univ. Leipzig, Math.-Nat. R. 27, 17–24 (1978)

    Google Scholar 

  • Claus, R., Hädge, D., Asperger, O., Fiebig, H., Kleber, H.-P.: Quantitative immunologische Bestimmungsmethode für Rubredoxin in Bakterienrohextrakten von Acinetobacter calcoaceticus. Z. Allg. Mikrobiol. 20, 95–103 (1980)

    PubMed  Google Scholar 

  • Kleber, H.-P., Aurich, H.: Einfluß von n-Alkanen auf die Synthese der Enzyme des Glyoxylatzyklus in Acinetobacter calcoaceticus. Z. Allg. Mikrobiol. 13, 473–480 (1973)

    PubMed  Google Scholar 

  • Kleber, H.-P., Schöpp, W., Aurich, H.: Verwertung von n-Alkanen durch einen Stamm von Acinetobacter calcoaceticus. Z. Allg. Mikrobiol. 13, 445–447 (1973)

    PubMed  Google Scholar 

  • Le Gall, J., Dragoni, N.: Dependence of sulphite reduction on a crystallized ferredoxin from Desulfovibrio gigas. Biochem. Biophys. Res. Commun. 23, 145–149 (1966)

    PubMed  Google Scholar 

  • Lovenberg, W., Sobel, B.: Rubredoxin — a new electron transfer protein from Cl. pasteurianum. Proc. Natl. Acad. Sci. USA 54, 193–199 (1965)

    PubMed  Google Scholar 

  • Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J.: Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265–275 (1951)

    PubMed  Google Scholar 

  • Meyer, T. E., Sharp, J. J., Bartsch, R. G.: Isolation and properties of rubredoxin from the photosynthetic green sulfur bacteria. Biochim. Biophys. Acta 234, 266–269 (1971)

    PubMed  Google Scholar 

  • Newman, D. J., Postgate, J. R.: Rubredoxin from a nitrogen-fixing variety of Desulfovibrio desulfuricans. Eur. J. Biochem. 7, 45–50 (1968)

    PubMed  Google Scholar 

  • Peterson, J. A.: Cytochrome content of two Pseudomonads containing mixed-function oxidase systems. J. Bacteriol. 103, 714–721 (1970)

    PubMed  Google Scholar 

  • Peterson, J. A., Basu, D., Coon, M. J.: Enzymatic ω-oxidation. I. Electron carriers in fatty acid and hydrocarbon hydroxylation. J. Biol. Chem. 241, 5162–5164 (1966)

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Claus, R., Asperger, O. & Kleber, HP. Influence of growth phase and carbon source on the content of rubredoxin in Acinetobacter calcoaceticus . Arch. Microbiol. 128, 263–265 (1980). https://doi.org/10.1007/BF00406170

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00406170

Key words

Navigation