Abstract
Long-chain alcohol dehydrogenase and longchain aldehyde dehydrogenase were induced in the cells of Candida tropicalis grown on n-alkanes. Subcellular localization of these dehydrogenases, together with that of acyl-CoA synthetase and glycerol-3-phosphate acyltransferase, was studied in terms of the metabolism of fatty acids derived from n-alkane substrates. Both longchain alcohol and aldehyde dehydrogenases distributed in the fractions of microsomes, mitochondria and peroxisomes obtained from the alkane-grown cells of C. tropicalis. Acyl-CoA synthetase was also located in these three fractions. Glycerol-3-phosphate acyltransferase was found in microsomes and mitochondria, in contrast to fatty acid β-oxidation system localized exclusively in peroxisomes. Similar results of the enzyme localization were also obtained with C. lipolytica grown on n-alkanes. These results suggest strongly that microsomal and mitochondrial dehydrogenases provide long-chain fatty acids to be utilized for lipid synthesis, whereas those in peroxisomes supply fatty acids to be degraded via β-oxidation to yield energy and cell constituents.
Similar content being viewed by others
References
Fukui, S., Kawamoto, S., Yasuhara, S., Tanaka, A., Osumi, M., Imaizumi, F.: Microbody of methanol-grown yeasts. Localization of catalase and flavin-dependent alcohol oxidase in the isolated microbody. Eur. J. Biochem. 59, 561–566 (1975)
Fukui, S., Tanaka, A.: Yeast peroxisomes. Trends Biochem. Sci. 4, 246–249 (1979a)
Fukui, S., Tanaka, A.: Peroxisomes of alkane-and methanol-grown yeasts. Metabolic functions and practical applications. J. Appl. Biochem. 1, 171–201 (1979b)
Gallo, M., Roche, B., Aubert, L., Azoulay, E.: Distribution des enzymes et des cytochromes de Candida tropicalis cultivé sur alcanes. Biochimie 55, 195–203 (1973)
Gallo, M., Roche, B., Azoulay, E.: Microsomal cytochromes of Candida tropicalis grown on alkanes. Biochim. Biophys. Acta 419, 425–434 (1976)
Gallo, M., Roche-Penverne, B., Azoulay, E.: Localisation de l'alcool deshydrogenase mitochondriale de Candida tropicalis. FEBS Lett. 46, 78–82 (1974)
Jones, C. L., Hajra, A. K.: The subcellular distribution of acyl CoA: dihydroxyacetone phosphate acyl transferase in guinea pig liver. Biochem. Biophys. Res. Commun. 76, 1138–1143 (1977)
Kawamoto, S., Nozaki, C., Tanaka, A., Fukui, S.: Fatty acid β-oxidation system in microbodies of n-alkane-grown Candida tropicalis. Eur. J. Biochem. 83, 609–613 (1978a)
Kawamoto, S., Tanaka, A., Yamamura, M., Teranishi, Y., Fukui, S., Osumi, M.: Microbody of n-alkane-grown yeast. Enzyme localization in the isolated microbody. Arch. Microbiol. 112, 1–8 (1977)
Kawamoto, S., Ueda, M., Nozaki, C., Yamamura, M., Tanaka, A., Fukui, S.: Localization of carnitine acetyltransferase in peroxisomes and in mitochondria of n-alkane-grown Candida tropicalis. FEBS Lett. 96, 37–40 (1978b)
Kawamoto, S., Yamada, T., Tanaka, A., Fukui, S.: Distinct subcellular localization of NAD-linked and FAD-linked glycerol-3-phosphate dehydrogenases in n-alkane-grown Candida tropicalis. FEBS Lett. 97, 253–256 (1979)
Krauzova, V. I., Sapozhnikova, G. P.: Alcohol dehydrogenase activity of Torulopsis candida during growth on intermediate oxidation products of glucose and hexadecane. Mikrobiologiya 48, 434–438 (1979)
Lebeault, J. M., Roche, B., Duvnjak, Z., Azoulay, E.: Alcool et aldéhyde-déshydrogénases particulaires de Candida tropicalis cultivé sur hydrocarbures. Biochim. Biophys. Acta 220, 373–385 (1970)
Mishina, M., Kamiryo, T., Tashiro, S., Hagihara, T., Tanaka, A., Fukui, S., Osumi, M., Numa, S.: Subcellular localization of two long-chain acyl-Coenzyme-A synthetases in Candida lipolytica. Eur. J. Biochem. 89, 321–328 (1978a)
Mishina, M., Kamiryo, T., Tashiro, S., Numa, S.: Separation and characterization of two long-chain acyl-CoA synthetases from Candida lipolytica. Eur. J. Biochem. 82, 347–354 (1978b)
Osumi, M., Fukuzumi, F., Teranishi, Y., Tanaka, A., Fukui, S.: Development of microbodies in Candida tropicalis during incubation in an n-alkane medium. Arch. Microbiol. 103, 1–11 (1975)
Osumi, M., Miwa, N., Teranishi, Y., Tanaka, A., Fukui, S.: Ultrastructure of Candida yeasts grown on n-alkanes. Appearance of microbodies and its relationship to high catalase activity. Arch. Microbiol. 99, 181–201 (1974)
Parekh, V. R., Traxler, R. W., Sobeck, J. M.: n-Alkane oxidation enzymes of a Pseudomonad. Appl. Environ. Microbiol. 33, 881–884 (1977)
Tanabe, I., Okada, J., Ono, H.: Isolation and determination of yeasts utilizing kerosene as sole source of carbon. Agric. Biol. Chem. 30, 1175–1182 (1966)
Tanaka, A., Yamamura, M., Kawamoto, S., Fukui, S.: Production of uricase by Candida tropicalis using n-alkane as a substrate. Appl. Environ. Microbiol. 34, 342–346 (1977)
Walker, J. D., Cooney, J. J.: Pathway of n-alkane oxidation in Cladosporium resinae. J. Bacteriol. 115, 635–639 (1973)
Yamashita, S., Numa, S.: Partial purification and properties of glycerophosphate acyltransferase from rat liver. Formation of 1-acylglycerol 3-phosphate from sn-glycerol 3-phosphate and palmityl Coenzyme A. Eur. J. Biochem. 31, 565–573 (1972)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Yamada, T., Nawa, H., Kawamoto, S. et al. Subcellular localization of long-chain alcohol dehydrogenase and aldehyde dehydrogenase in n-alkane-grown Candida tropicalis . Arch. Microbiol. 128, 145–151 (1980). https://doi.org/10.1007/BF00406151
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00406151