Summary
A detailed procedure of a new photometric assay for total diamines and polyamines in human urine using soybean seedling amine oxidase (SSAO) as an enzyme reagent is described. It is based on the unique substrate specificity of SSAO that the enzyme is active toward all diamines and polyamines. The amines were purified from urine by cation-exchange chromatography and incubated with SSAO. Hydrogen peroxide formed in the oxidase reaction was measured photometrically by coupling 4-aminoantipyrine with phenol in the presence of peroxidase. For its simplicity and sensitivity, our method seems useful for routine clinical investigation. The data obtained from normal subjects and patients of various cancers are presented to validate the present method.
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Abbreviations
- SSAO:
-
soybean seedling amine oxidase
- Put:
-
putrescine
- Cad:
-
cadaverine
- Spd:
-
spermidine
- Spm:
-
sprmine
References
Bardsley WG, Hill CM, Lobley RW (1970) A reinvestigation of the substrate specificity of pig kidney diamine oxidase. Biochem. J. 117:169–176
Bonsnes RW, Taussky HH (1945) The colorimetric determination of creatinine by the Jaffe reaction. J Biol Chem 158:581–591
Campbell RA, Morris DR, Bartos D, Daves GD, Bartos F (eds) (1978) Advances in polyamine research, Vols 1 and 2. Raven Press, New York
Emerson E (1943) The condensation of aminoantipyrine. II. A new color test for phenolic compounds. J Org Chem 8:417–428
Fujita K, Nagatsu T, Maruta K, Ito M, Senba H, Miki K (1976) Urinary putrescine, spermidine, and spermine in human blood and solid cancers and in a experimental gastric tumor of rats. Cancer Res 36:1320–1324
Hill JM (1971) Diamine oxidase (pea seedling). In: Tabor H, Tabor CW (eds) Methods in enzymology, Vol. XVII, Part B. Academic Press, New York, pp 730–735
Møller KM, Ottolenghi P (1966) The oxidation of o-dianisidine by H2O2 and peroxidase at neutral pH. Compt Rend Trav Lab Carlsberg 35:369–389
Perry TL, Hansen S, Hestrin M, Macintyre L (1965) Exogenous urinary amines of plant origin. Clin Chim Acta 11:24–34
Rodnight R (1956) Separation and characterization of urinary indoles resembling 5-hydroxytryptamine and tryptamine. Biochem. J 64:621–626
Russell DH (ed) (1973) Polyamines in normal and neoplastic growth. Raven Press. New York
Russell DH, Durie BGM (1978) Polyamines as biochemical markers of normal and malignant growth. Raven Press, New York
Russell DH, Levy CC, Schimp SC, Hawk IA (1971) Urinary polyamines in cancer patients. Cancer Res 31:1555–1558
Russell DH, Durie BGM, Salmon SE (1975) Polyamines as predictors of success and failure in cancer chemotherapy. Lancet 2:797–799
Smith JK (1967) The purification and properties of placental histaminase. Biochem J 103:110–119
Smith TA (1972) Purification and properties of the polyamine oxidase of barley plants. Phytochemistry 11:899–910
Smith TA (1977) Further properties of the polyamine oxidase from oat seedlings. Phytochemistry 16:1647–1649
Suzuki O, Yagi K (1977) A fluorometric assay for β-phenylethylamine in human urine. Clin Chim Acta 78:401–410
Suzuki O, Noguchi E, Yagi K (1977) Monoamine oxidase in developing chick retina. Brain Res 135:305–313
Suzuki Y (1973) Some properties of the amine oxidase of soybean seedlings. Plant Cell Physiol 14:413–417
Tabor CW, Tabor H, Rosenthal SM (1954) Purification of amine oxidase from beef plasma. J Biol Chem 208:645–661
Tipton KF, Youdim MBH (1976) Assay of Monoamine oxidase. In: Wolstenholme GEW, Knight J (eds) Monoamine oxidase and its inhibition. Elsevier, Amsterdam, pp 393–403
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Matsumoto, T., Suzuki, O., Katsumata, Y. et al. A new enzymatic assay for total diamines and polyamines in urine of cancer patients. J Cancer Res Clin Oncol 100, 73–84 (1981). https://doi.org/10.1007/BF00405904
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DOI: https://doi.org/10.1007/BF00405904