Summary
Zygotes of Saccharomyces cerevisiae that are heteroplasmic for mitochondrial alleles produce diploid progeny that are homoplasmic for one allele or the other, judged by the criterion that upon further subcloning they produce daughter cells of only one phenotype or the other. Here we show that when such cells are subjected to strong selection for the missing allele, it cannot be detected, so that it is probably not present in even a single copy.
References
Backer J (1980) The origin of mutant cells: the mechanisms by which Saccharomyces cerevisiae produce cells homoplasmic for new mitochondrial mutations. PhD Thesis, The Ohio State University
Birky CW Jr (1973) Genetics 74:421–432
Birky CW Jr (1978) Ann Rev Genet 12:471–512
Birky CW Jr (1983a) Int Rev Cytol, Suppl 15:49–89
Birky CW Jr (1983b) Science 222:468–475
Birky CW Jr, Acton AA, Dietrich R, Carver M (1982) Mitochondrial transmission genetics: replication, recombination, and segregation of mitochondrial DNA and its inheritance in crosses. In: Attardi G, Borst P, Slonimski PP (eds) Mitochondrial Genes. Cold Spring Harbor Laboratory, New York, pp 333–348
Dujon B (1982) Mitochondrial genetics and functions. In: Strathern JN, Jones EW, Broach JR (eds) The molecular biology of the yeast Saccharomyces. Life cycle and inheritance. Cold Spring Harbor Laboratory, New York, pp 505–635
Knight JA (1980) Genetics 94:69–92
Thrailkill KM, Birky CW Jr, Luckemann G, Wolf K (1980) Genetics 96:237–262
Treat-Clemmons LG, Birky CW Jr (1983) Curr Genet 7:489–492
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Lewis, J.E., Birky, C.W. Homoplasmic yeast cells contain no selectable “hidden” mitochondrial alleles. Curr Genet 8, 81–84 (1984). https://doi.org/10.1007/BF00405436
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DOI: https://doi.org/10.1007/BF00405436