Summary
Prototrophic hybrids have been obtained by the fusion of auxotrophic haploid strains of the two yeasts Yarrowia (Saccharomycopsis) lipolytica and Kluyveromyces lactis. The hybrid fusants had a colonial morphology intermediate between that of the two parent strains, were uninucleate, and contained an approximately diploid amount of DNA per cell. The growth rates of all the fusants on a minimal glucose medium were slower than those of the two parents. Two of the fusants studied could utilise a novel range of carbon sources.
All of these data suggested that the hybrids contained a diploid nucleus formed by the fusion of the two haploid parental nuclei. However, analytical CsCl density gradient centrifugation demonstrated that the nuclear DNA of the fusants was derived almost entirely from the Y. lipolytica parent. Moreover, an examination of the protein constitution of the fusants by two-dimensional gel electrophoresis showed that their protein patterns were indistinguishable from that of Y. lipolytica. Two possible mechanisms for the formation of a diploid nucleus containing DNA derived almost entirely from one of the haploid parents are discussed.
Similar content being viewed by others
References
Dutcher SK (1981) Mol Cell Biol 1:245–253
Elliott SG, McLaughlin CS (1978) Proc Natl Acad Sci USA 75:4384–4388
Ferenczy L (1981) Microbial protoplast fusion. In: Glover SW, Hopwood DA (eds) Genetics as a tool in microbiology (31st Symp Soc Gen). University Press, Cambridge, pp 1–34
Gaber RF, Leonard TJ (1981) Nature 291:342–344
Goodey AR, Tubb RS (1982) J Gen Microbiol 128:2615–2620
Leonard TJ, Dick S, Gaber RF (1978a) Genetics 88:13–26
Leonard TJ, Gaber RF, Dick S (1978b) Genetics 89:635–654
Lodder J (1970) The Yeasts. North-Holland, Amsterdam
Ludwig JR, Oliver SG, McLaughlin CS (1977) Mol Gen Genet 158:117–122
O'Farrell PH (1975) J Biol Chem 250:4007–4021
Oliver SG, Williamson DH (1976) Mol Gen Genet 146:253–259
Peberdy JF (1980) Enzyme Microbiol Technol 2:23–29
Provost A, Bourguignon C, Fournier P, Ribert AM, Heslot H (1978) FEMS Letts 3:309–312
Spata L, Weber H (1980) A study of protoplast fusion and parasexual hybridisation of alkane-utilizing yeasts. In: Ferenczy L, Farkas GL (eds) Advances in protoplast research. Pergamon Press, Oxford, pp 131–137
Specht CA, DiRusso CC, Novotny CP, Ullrich RC (1982) Anal Biochem 119:158–163
Stahl U (1978) Mol Gen Genet 160:111–113
Vo K-P, Miller MJ, Geiduschek EP, Nielsen C, Olson A, Xuong NH (1981) Anal Biochem 112:258–271
Weiss MC, Green H (1967) Proc Natl Acad Sci (USA) 58:1104–1111
Williamson DH, Fennell DJ (1976) Meth Cell Biol 12:335–351
Wilson JJ, Khachatourians GG, Ingledew WM (1982) Mol Gen Genet 186:95–100
Windass JD, Worsey MJ, Pioli EM, Pioli D, Barth PT, Byrom D,Powell K, Senior PJ (1980) Nature 287:396–401
Zamb TJ, Roth R (1977) Yeast Newsletter 25:140–142
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Groves, D.P., Oliver, S.G. Formation of intergeneric hybrids of yeast by protoplast fusion of Yarrowia and Kluyveromyces species. Curr Genet 8, 49–55 (1984). https://doi.org/10.1007/BF00405432
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00405432