Skip to main content
SpringerLink
Log in

glnA mutations define the structural gene for glutamine synthetase in Aspergillus

  • Original Articles
  • Published:
Current Genetics Aims and scope Submit manuscript

Summary

Five allelic glutamine auxotrophs of Aspergillus have been assayed for glutamine synthetase activity. The amounts of glutamine synthetase activity present in these strains were proportional to their ability to grow in the absence of L-glutamine, whereas the amount of γ-glutamyl transferase did not correlate with the amount of synthetase or with the growth properties. Two out of this sample of mutants have been shown to have a glutamine synthetase with a K m for L-glutamate which differs from the wild type.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Arst HN Jr (1981) Symp Soc Gen Microbiol 31:131–160

    Google Scholar 

  • Arst HN Jr, Brownlee AG, Cousen SA (1982) Curr Genet 6:245–257

    Google Scholar 

  • Bradford MM (1976) Anal Biochem 72:248–254

    Article  CAS  PubMed  Google Scholar 

  • Clutterbuck JA (1974) Aspergillus nidulans. In: King RC (ed) Handbook of genetics, vol 1. Plenum Press, New York, pp 447–510

    Google Scholar 

  • Cove DJ (1966) Biochim Biophys Acta 113:51–56

    PubMed  Google Scholar 

  • Drainas C, Kinghorn JR, Pateman JA (1977) J Gen Microbiol 98:493–501

    Google Scholar 

  • Dunn E, Pateman JA (1972) Heredity 29:129

    Google Scholar 

  • Dunn-Coleman NS, Garrett RH (1980) Mol Gen Genet 179:25–32

    PubMed  Google Scholar 

  • Fincham JRS, Coddington A (1963) J Mol Biol 6:361–373

    PubMed  Google Scholar 

  • Ginsburg A, Stadtman ER (1973) In: Prusiner S, Stadtman ER (eds) The enzymes of glutamine metabolism. Academic Press, New York, pp 9–44

    Google Scholar 

  • MacDonald DW (1982) Curr Genet 6:203–208

    Google Scholar 

  • Magasanik B (1982) Annu Rev Genet (1982) 16:135–168

    Google Scholar 

  • Marzluf GA (1981) Microbiol Rev 45:437–461

    PubMed  Google Scholar 

  • McFarland N, McCarter L, Artz S, Kustu S (1981) Proc Natl Acad Sci USA 78:2135–2139

    PubMed  Google Scholar 

  • Palacios R (1976) J Biol Chem 251:4781–4791

    Google Scholar 

  • Stewart GR, Mann AF, Fentem PA (1980) In: Miflin BJ (ed) The biochemistry of plants, vol V. Amino acids and their derivatives. Academic Press, London New York, pp 271–327

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Genetics, University of Cambridge, Downing Street, C132 3EH, Cambridge, UK

    E. V. Cornwell & D. W. MacDonald

Authors
  1. E. V. Cornwell
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. D. W. MacDonald
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Cornwell, E.V., MacDonald, D.W. glnA mutations define the structural gene for glutamine synthetase in Aspergillus . Curr Genet 8, 33–36 (1984). https://doi.org/10.1007/BF00405429

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00405429

Key words

Search

Navigation