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Fluorescence microphotolysis

Diffusion measurements in single cells

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Abstract

Fluorescence microphotolysis is a versatile method for diffusion measurements in single cells and other microscopic systems. A recent development, continuous fluorescence microphotolysis, extends diffusion measurements to ensembles of a few hundred fluorophores. Cell surface membranes are frequently considered as 2-dimensional fluids. Membrane proteins, dispersed in a fluid bimolecular lipid layer, are expected to be highly mobile in the membrane plane. Such expectations are met by artificial bilayers but not always by cell membranes. As first observed for erythrocytes and subsequently for many other cells translational mobility of membrane proteins can be severely restricted. Frequently, integral membrane proteins have mobile and immobile fractions. In order to account for this duality it has been suggested that cell surface membranes are made up of two layers with opposing dynamic properties: a fluid lipidprotein bilayer, the “membrane skin”, and a rather static protein network, the “membrane skeleton”.

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References

  1. Singer, S.J., Nicolson, G.L.: Science 175, 720 (1972)

    Google Scholar 

  2. Adam, G., Delbrück, M., in: Structural Chemistry and Molecular Biology (Rich, A., Davidson, N., eds.). San Francisco-London: Freeman 1968

    Google Scholar 

  3. Cuatrecasas, P.: Ann. Rev. Biochem. 43, 169 (1974)

    Google Scholar 

  4. Liebman, P.A., Pugh, E.N. Jr.: Vision Res. 19, 375 (1979)

    Google Scholar 

  5. Hackenbrock, C.R.: Trends Biochem. Sci. 6, 151 (1981)

    Google Scholar 

  6. Skulachev, V.P.: Biochim. Biophys. Acta 604, 297 (1980)

    Google Scholar 

  7. Peters, R.: ibid. 330, 53 (1973)

    Google Scholar 

  8. Peters, R., et al: Pflügers Arch. 347, 72 (1974)

    Google Scholar 

  9. Peters, R., et al.: Biochim. Biophys. Acta 367, 282 (1974)

    Google Scholar 

  10. Edidin, M., et al.: Science 191, 466 (1976)

    Google Scholar 

  11. Jacobson, K., et al.: J. Supramol. Struct. 5, 566 (1977)

    Google Scholar 

  12. Axelrod, D., et al.: Biophys. J. 16, 1056 (1976)

    Google Scholar 

  13. Peters, R.: Cell Biol. Int. Rep. 5, 1 733 (1981)

    Google Scholar 

  14. Edidin, M., in: Membrane Structure, p. 39 (Finean, J.B., Mitchell, R.H., eds.). Amsterdam: Elsevier/North-Holland Biomedical Press 1981

    Google Scholar 

  15. Webb, W.W., et al.: Biochem. Soc. Symp. 46, 191 (1981)

    Google Scholar 

  16. Jacobson, K., Wojicieszyn, J.: Comments Mol. Cell Biophys. 1, 189 (1981)

    Google Scholar 

  17. Schlessinger, J.: Trends Biochem. Sci. 5, 210 (1980)

    Google Scholar 

  18. Cherry, R.: Biochim. Biophys. Acta 559, 289 (1979)

    Google Scholar 

  19. Smith, B.A., McConnell, H.M.: Proc. Nat. Acad. Sci. USA 75. 2759 (1978)

    Google Scholar 

  20. Smith, B.A., et al.: ibid. 76, 5641 (1979)

    Google Scholar 

  21. Smith, L.M., et al: Biophys. J. 33, 73 (1981)

    Google Scholar 

  22. Wegener, W., Rigler, R.: Int. Workshop on the Biological Applications of Photobleaching Techniques, Chapel Hill, North Carolina, USA 1981

  23. Peters, R., Richter, H.-P.: Dev. Biol. 86, 285 (1981)

    Google Scholar 

  24. Peters, R., et al.: Proc. Nat. Acad. Sci. USA 78, 962 (1981)

    Google Scholar 

  25. Brünger, A.: Thesis Techn. Univ. München 1982

  26. Sheetz, M.P., Koppel, D.E.: Proc. Nat. Acad. Sci. USA 76, 3314 (1979)

    Google Scholar 

  27. Schlessinger, F., et al.: Science 195, 307 (1977)

    Google Scholar 

  28. Jacobson, K., et al.: Exp. Cell Res. 116, 179 (1978)

    Google Scholar 

  29. Wolf, D.E., et al.: Proc. Nat. Acad. Sci. USA 77, 2043 (1981)

    Google Scholar 

  30. Koppel, D.E., Sheetz, M.P.: Nature 293, 159 (1981)

    Google Scholar 

  31. Wey, C.-L., et al.: Biophys. J. 33, 225 (1981)

    Google Scholar 

  32. Saffman, P.G., Delbrück, M.: Proc. Nat. Acad. Sci. USA 72, 3111 (1975)

    Google Scholar 

  33. Saffman, P.G.: J. Fluid Mechn. 73, 593 (1976)

    Google Scholar 

  34. Hughes, B.D., et al.: ibid. 110, 349 (1981)

    Google Scholar 

  35. Peters, R., Cherry, R.J.: Proc. Nat. Acad. Sci. USA 79, 4317 (1982)

    Google Scholar 

  36. Cherry, R.J., et al.: FEES Lett. 80, 465 (1977)

    Google Scholar 

  37. Cherry, R.J., et al.: J. Mol. Biol. 121, 283 (1978)

    Google Scholar 

  38. Cherry, R.J., Godfrey, R.E.: Biophys. J. 36, 257 (1981)

    Google Scholar 

  39. Heyn, M.P., et al.: Biochemistry 20, 840 (1981)

    Google Scholar 

  40. Henderson, R., Unwin, P.N.T.: Nature 257, 28 (1975)

    Google Scholar 

  41. Einstein, A.: Ann. Physik 19, 286 (1906)

    Google Scholar 

  42. Freire, E., Snyder, B.: Biophys. J. 37, 617 (1982)

    Google Scholar 

  43. Poo, M.-H., Cone, R.A.: Nature 247, 438 (1974)

    Google Scholar 

  44. Wu, E.-S., et al.: Biochemistry 17, 5543 (1978)

    Google Scholar 

  45. Vaz, W.L.C., et al.: Proc. Nat. Acad. Sci. USA 76, 5645 (1979)

    Google Scholar 

  46. Smith, L.M., et al.: Biochemistry 18, 2256 (1979)

    Google Scholar 

  47. Wu, E.-S., Yang, C.S.: Fed. Proc. 39, 1990 (1981)

    Google Scholar 

  48. Vaz, W.L.C., et al.: Biochemistry 20, 1392 (1981)

    Google Scholar 

  49. Chang, C.-H., et al.: J. Biochem. 90, 997 (1981)

    Google Scholar 

  50. Golan, D.E., Veatch, W.: Proc. Nat. Acad. Sci. USA 77, 2537 (1980)

    Google Scholar 

  51. Golan, D.E., Veatch, W.: Biophys. J. 37, 177a (1982)

    Google Scholar 

  52. Kapitza, H.-G., Sackmann, E.: Biochim. Biophys. Acta 595, 56 (1980)

    Google Scholar 

  53. Thompson, N.L., Axelrod, D.: ibid. 597, 155 (1980)

    Google Scholar 

  54. Golan, D.E., Veatch, W.: Int. Workshop on the Biol. Applications of Photobleaching Techniques, Chappel Hill, North Carolina, USA 1981

  55. Steck, T.L.: J. Cell Biol. 62, 1 (1974)

    Google Scholar 

  56. Marchesi, V.T.: J. Membr. Biol. 51, 101 (1979)

    Google Scholar 

  57. Lux, S.E.: Semin. Hematol. 16, 21 (1979)

    Google Scholar 

  58. Branton, D., et al.: Cell 24, 24 (1981)

    Google Scholar 

  59. Cherry, R.J., et al.: Nature 263, 383 (1976)

    Google Scholar 

  60. Nigg, E.A., Cherry, R.J.: Proc. Nat. Acad. Sci. USA 77, 4702 (1980)

    Google Scholar 

  61. Sheetz, M.P., et al.: Nature 285, 510 (1980)

    Google Scholar 

  62. Fowler, V., Branton, D.: ibid. 268, 23 (1977)

    Google Scholar 

  63. Schindler, M., et al.: Proc. Nat. Acad. Sci. USA 77, 1457 (1980)

    Google Scholar 

  64. Cohen, C.M., Branton, D.: Trends Biochem Sci. 6, 266 (1981)

    Google Scholar 

  65. Eldridge, C.A., et al.: Biochemistry 19, 2075 (1980)

    Google Scholar 

  66. Schlessinger, J., et al.: Proc. Nat. Acad. Sci. USA 75, 5353 (1978)

    Google Scholar 

  67. Dragsten, P., et al.: ibid. 76, 5163 (1979)

    Google Scholar 

  68. Weatherbee, J.A.: Int. Rev. Cytol., Suppl. 12, 113 (1981)

    Google Scholar 

  69. Loor, F.: Cell Surf. Rev. 7, 255 (1981)

    Google Scholar 

  70. Schlessinger, J., et al.: Proc. Nat. Acad. Sci. USA 74, 1110 (1977)

    Google Scholar 

  71. Huxley, H.E. Nature 243, 445 (1973)

    Google Scholar 

  72. Singer, S.J.: Ann. Rev. Biochem. 43, 805 (1974)

    Google Scholar 

  73. Evans, E.A., Hochmuth, R.M.: J. Membr. Biol. 30, 351 (1977)

    Google Scholar 

  74. Mescher, M.F., et al.: Nature 289, 139 (1981)

    Google Scholar 

Download references

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  1. Max-Planck-Institut für Biophysik, D-6000, Frankfurt

    R. Peters

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Peters, R. Fluorescence microphotolysis. Naturwissenschaften 70, 294–302 (1983). https://doi.org/10.1007/BF00404836

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  • DOI: https://doi.org/10.1007/BF00404836

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