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H-2-specific antibodies induced by injection of syngeneic leukemia cells

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Abstract

AKR/J mice immunized with several syngeneic leukemia cells contained antibodies in their sera which reacted with certain AKR leukemia cell lines, depending on their H-2 expression, and precipitated H-2K antigens from lysates of leukemia cells. Precipitation of H-2K was not due to virus-specific antibodies: it could not be blocked by prior absorption with H-2-negative leukemias, but was blocked by certain allogeneic lymphocytes. Tumor-specific H-2K antibodies did not react with H-2K from normal AKR lymphocytes either on the cell surface or after detergent solubilization; however, they did react with H-2K from mitogen-activated AKR and BALB.K lymphoblasts. Since both these latter cells were also lysed by AKR-Gross/MuLV-specific and H-2Kk-restricted cytotoxic T lymphocytes, we consider the possibility that antibodies detecting conformational alterations induced in H-2Kk molecules by viral association may be present in syngeneic AKR antileukemia sera.

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Abbreviations

GCSA:

Gross-virus-induced cell-surface antigen

MCF:

mink cell focus-forming virus

MuLV:

murine leukemia virus

Th:

T helper

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  1. Institut für Zellbiologie (Tumorforschung), Universität Essen (GH), Hufelandstrasse 55, D-4300, Essen 1, Germany

    Wilhelm Schmidt

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Schmidt, W. H-2-specific antibodies induced by injection of syngeneic leukemia cells. Immunogenetics 25, 215–221 (1987). https://doi.org/10.1007/BF00404690

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  • DOI: https://doi.org/10.1007/BF00404690

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