Summary
The effect of photochemotherapy on skin collagen metabolism was investigated in 57 patients with psoriasis. Twenty-eight patients were treated with trioxsalen baths and UVA irradiation, nine with ditranol and UVB irradiation, and 20 untreated psoriasis patients served as controls. No significant changes were found between the treated groups and the control group in urinary hydroxyproline excretion, skin hydroxyproline content, or in the activities of two enzymes catalyzing collagen biosynthesis, prolyl hydroxylase, and galactosylhydroxylysyl glucosyltransferase in the skin. When the same parameters were compared with the cumulative doses of UVA irradiation, skin prolyl hydroxylase activity slightly decreased with increasing UVA doses, whereas no changes were found in skin hydroxyproline content or galactosylhydroxylysyl glucosyltransferase activity. It was concluded that long-term local PUVA therapy does not significantly alter collagen metabolism in the skin.
Zusammenfassung
Die Wirkung der Photochemotherapie auf den Kollagenmetabolismus der Haut wurde an Psoriasis-Patienten (n=57) untersucht.
Achtundzwanzig Patienten wurden mit Trioxsalen-Bädern und UVA-Irradiation, neun mit Ditranol und UVB-Irradiation behandelt. Zwanzig Probanden dienten als Kontrollgruppe. Es wurden keine Unterschiede zwischen den Behandelten und der Kontrollgruppe festgestellt (Hydroxyprolinkonzentration im Urin, Hydroxyprolinkonzentration in der Haut und Prolinhydroxylase sowie Galactosyl-Hydroxylysyl-Glucosyltransferaseaktivität).
Dieselben Parameter wurden bei der Anwendung mit kumulativen Dosen von UVA-Irradiationen verglichen. Dabei nahm die Aktivität der Prolinhydroxylase der Haut je nach der Größe der UVA-Dosen ab, hingegen waren keine Änderungen des Hydroxyprolingehaltes der Haut oder der Galactosyl-Hydroxylysyl-Glucosyltransferase nachzuweisen. Langfristige lokale PUVA-Therapie beeinflußt somit nicht den Kollagenmetabolismus der Haut.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Baden HP, Parrington JM, Delhanty JDA, Pathak MA (1972) DNA synthesis in normal and xeroderma pigmentosum fibroblasts following treatment with 8-methoxypsoralen and long-wave ultraviolet light. Biochim Biophys Acta 262:247–255
Blumenkrantz N, Weismann K, Asboe-Hansen G (1979) Lack of dermal changes in uninvolved skin after photochemotherapy of psoriasis: biochemical studies. Acta Derm Venereol (Stockh) 59:149–151
Everett MA, Yergers E, Sayre RM, Olson RL (1966) Penetration of epidermis by ultraviolet rays. Photochem Photobiol 5:533–542
Fleckman PH, Jeffrey JJ, Eisen AZ (1973) A sensitive microassay for prolyl hydroxylase: activity in normal and psoriatic skin. J Invest Dermatol 60:46–52
Gazith J, Schalla W, Bauer E, Schaefer H (1978) 8-methoxypsoralen (8-MOP) in human skin: penetration kinetics. J Invest Dermatol 71:126–130
Hannuksela M, Karvonen J (1978) Trioxsalen baths plus UVA effective and safe in the treatment of psoriasis. Br J Dermatol 99:703–707
Kammerau B, Klebe U, Zesch A, Schaefer H (1976) Penetration permeation and resorption of 8-methoxypsoralen. Arch Dermatol Res 255:31–42
Kivirikko KI, Laitinen O, Prockop DJ (1967) Modifications of a specific assay for hydroxyproline in urine. Anal Biochem 19:249–255
Kivirikko KI (1970) Urinary excretion of hydroxyproline in health and disease. In: Hall DA, Jackson DS (eds) International review of connective tissue research, Vol 5. Academic Press, New York, pp 93–163
Kivirikko KI, Risteli L (1976) Biosynthesis of collagen and its alterations in pathological states. Med Biol 54:159–186
Kuutti ER, Tuderman L, Kivirikko KI (1975) Human prolyl hydroxylase. Purification, partial characterization, and preparation of antiserum to the enzyme. Eur J Biochem 57:181–188
Kuutti-Savolainen ER, Kero M (1979) Enzymes of collagen biosynthesis in skin and serum in dermatological diseases. I. Enzymes of the skin. Clin Chim Acta 96:43–51
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:365–375
Myllylä R, Risteli L, Kivirikko KI (1975) Assay of collagen galactosyltransferase and collagen glucosyltransferase activities and preliminary characterization of enzymic reactions with transferases from chick-embryo cartilage. Eur J Biochem 52:401–410
Pierard GE, de la Brassinne M, Lapière ChM (1977) Effects of long-term photochemotherapy of the dermis. J Invest Dermatol (abstract) 68:249–250
Pohl J, Christophers E (1979) Photoinactivation of skin fibroblasts by fractionated treatment with 8-methoxypsoralen and UVA. J Invest Dermatol 73:176–179
Prockop DJ, Kivirikko KI, Tuderman L, Guzman NA (1979) The biosynthesis of collagen. N Engl J Med 301:77–85
Risteli J, Tuderman L, Kivirikko KI (1976) Intracellular enzymes of collagen biosynthesis in rat liver as a function of age and in hepatic injury induced by dimethylnitrosamine. Purification of rat prolyl hydroxylase and comparison of changes in prolyl hydroxylase activity with changes in immunoreactive prolyl hydroxylase. Biochem J 158:369–376
Uitto J, Halme J, Hannuksela M, Peltokallio P, Kivirikko KI (1969) Protocollagen proline hydroxylase activity in the skin of normal human subjects and of patients with scleroderma. Scand J Clin Lab Invest 23:241–247
Uitto J, Hannuksela M, Rasmussen OG (1970) Protocollagen proline hydroxylase activity in scleroderma and other connective tissue diseases. An Clin Res 2:235–239
Uitto J (1971) Collagen biosynthesis in human skin. A review with emphasis on scleroderma. Ann Clin Res 3:250–258
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Väätäinen, N., Oikarinen, A. & Kuutti-Savolainen, E.R. The effects of long-term local PUVA treatment on collagen metabolism in human skin. Arch Dermatol Res 269, 99–104 (1980). https://doi.org/10.1007/BF00404464
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00404464