Summary
Rat hair follicles obtained from the skin of 12-day-old animals were cultured in the presence of 20% fetal calf serum and compared with those cultured in the presence of 20% rat serum obtained from animals aged 12–14, 18–20 days, and 1 year.
Follicles cultured in the presence of fetal calf or 12–14-day rat serum showed no morphological change after 2 days of culture. Follicles cultured in the presence of 18–20 day serum or adult serum for 2 days showed greatly altered morphology, the dermal papilla degenerated, keratinization of hair cells occurred, and an enlarged outer sheath developed. The cells in this sheath contained many filaments which were periodic acid-Schiff positive. These changes are compared with those occurring during the catagen phase of the hair cycle in vivo.
Zusammenfassung
Haarfollikel von 12 Tage alten Ratten wurden mit 20% fetalem Kalbsserum zur Kultur angesetzt und mit Kulturen verglichen, denen 20%iges Rattenserum von Tieren im Alter von 12–14, 18–20 Tagen und 1 Jahr, zugesetzt wurde.
Follikelkulturen, die mit fetalem Kalbsserum oder 12–14 Tage altem Rattenserum versetzt waren, zeigten keine morphologische Veränderungen nach 2 Tagen kulturellen Wachstums. Follikel, deren Kultur mit Serum von 18–20 Tage alten oder erwachsenen Tieren versetzt waren, zeigten nach 2 Tagen ausgeprägte morphologische Veränderungen. Die dermale Papille war degeneriert. Es entwickelte sich eine Keratinisation der Haarzellen und eine Verbreiterung der äußeren Scheide. Die Zellen in dieser Scheide enthielten viele Filamente, die PAS-positiv waren. Diese Veränderungen wurden mit solchen verglichen, die sich während der Katagenphase des Haarcyclus in vivo entwickelten.
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Frater, R. The effect of rat serum on the morphology of rat hair follicles in tissue culture. Arch Dermatol Res 269, 13–20 (1980). https://doi.org/10.1007/BF00404453
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DOI: https://doi.org/10.1007/BF00404453