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Immunohistochemical studies of basal cell carcinomas transplanted into nude mice

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Summary

Xenografting into nude mice forms a system for analysis of human tissues under experimental conditions. In this study, normal skin samples and basal cell carcinomas were investigated, prior to and after transplantation, using immunofluorescence methods with antibodies against keratins, laminin, and collagen type IV. Three groups of transplants were studied: (a) intact tissue samples, (b) human epithelium (either normal or neoplastic) recombined with normal human dermis and, (c) human epithelium recombined with normal mouse dermis. Transplants recovered after 3 weeks showed the following characteristics. 1) The xenograft system was satisfactory in terms of host survival and rate of successful tissue recovery except for recombinants between human epithelium and mouse dermis. 2) Intact and recombined samples of normal skin retained their preexisting patterns of architecture, cytodifferentiation, and basement membrane staining. 3) Solid nonfibrosing basal cell carcinomas showed altered architecture and differentiation of both the epithelium and the basement membrane zone after transplantation: the solid tumor pattern changed towards spreading of tumor cells, a more squamous differentiation pattern was apparent and was confirmed by reactivity with antibodies against large keratins. Discontinuities of the basement membrane zone were detected with antibodies against laminin and collagen type IV. These changes were seen in both intact and recombined tumor transplants.

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References

  1. Bauer EZ, Gordon JM, Reddick ME, Eisen AZ (1977) Quantitation and immunocytochemical localization of human skin collagenase in basal cell carcinoma. J Invest Dermatol 69:363–367

    Article  PubMed  Google Scholar 

  2. De Moraga JM, Winkelmann RK, Jordon RE (1970) Immunofluorescence of epithelial skin tumors. Cancer 25:1404–1407

    PubMed  Google Scholar 

  3. Doran TI, Vidrich A, Sun T-T (1980) Intrinsic and extrinsic regulation of the differentiation of skin, corneal and esophageal epithelial cells. Cell 22:17–25

    Article  PubMed  Google Scholar 

  4. Franke WW, Schiller DL, Moll R, Winter A, Schmid E, Engelbrecht I, Denk H, Krepler R, Platzer B (1981) Diversity of cytokeratins. Differentiation specific expression of cytokeratin polypeptides in epithelial cells and tissues. J Mol Biol 153:933–959

    PubMed  Google Scholar 

  5. Fuchs E (1981) Evolution and complexity of the genes encoding the keratins of human epidermal cells. J Invest Dermatol 81:141s-144s

    Google Scholar 

  6. Grimwood RE, Huff JC, Harbell JW, Clark RAF (1984) Fibronectin in basal cell epithelioma: sources and significance. J Invest Dermatol 82:145–149

    Article  PubMed  Google Scholar 

  7. Grimwood RE, Johnson CA, Ferris CF, Mercill DB, Mellette JR, Huff JC (1985) Transplantation of human basal cell carcinomas to athymic nude mice. Cancer 56:519–523

    PubMed  Google Scholar 

  8. Grimwood RE, Ferris CF, Mercill DB, Huff JC (1986) Proliferating cells of human basal cell carcinoma are located on the periphery of tumor nodules. J Invest Dermatol 86:191–194

    Article  PubMed  Google Scholar 

  9. Haftek M, Ortonne JP, Staquet MJ, Viac J, Thivolet J (1981) Normal and psoriatic human skin grafts on “nude” mice: morphological and immunochemical studies. J Invest Dermatol 76:48–52

    Article  PubMed  Google Scholar 

  10. Holmstrup P, Dabelsteen E, Reibel J, Harder F (1981) Normal keratinized mucosa transplants in nude mice. Acta Odontol Scand 39:187–193

    PubMed  Google Scholar 

  11. Hundeiker M (1983) Die Basaliome. Aus der Sicht der Histologie. Ber Pathol 98:725–795

    Google Scholar 

  12. Jacobs GH, Rippey JJ, Altini M (1982) Prediction of aggressive behavior in basal cell carcinoma. Cancer 49:553–537

    PubMed  Google Scholar 

  13. Kallioinen M, Autio-Harmainen H, Dammert K, Risteli J, Risteli L (1984) Discontinuity of the basement membrane in fibrosing basocellular carcinomas and basosquamous carcinomas of the skin: an immunohistochemical study with human laminin and type IV collagen antibodies. J Invest Dermatol 82:248–251

    Article  PubMed  Google Scholar 

  14. Lever WF, Schaumburg-Lever G (1983) Histopathology of the skin, 6th edn. Lippincott, Philadelphia, pp 562–575

    Google Scholar 

  15. Liotta LA, Rao CN, Barsky SH (1983) Tumor invasion and the extracellular matrix. Lab Invest 49:636–649

    PubMed  Google Scholar 

  16. Löning T, Staquet MJ, Thivolet J, Seifert G (1980) Keratin polypeptides distribution in normal and diseased human epidermis and oral mucosa. Immunohistochemical study on unaltered epithelium and inflammatory premalignant and malignant lesions. Virchows Arch [A] 388:273–288

    Google Scholar 

  17. Mackenzie IC, Hill MW (1981) Maintenance of regionally specific patterns of cell proliferation and differentiation in transplanted skin and oral mucosa. Cell Tissue Res 219:597–607

    Article  PubMed  Google Scholar 

  18. Mackenzie IC, Hill MW (1984) Connective tissue influences on patterns of epithelial architecture and keratinization in skin and oral mucosa of the adult mouse. Cell Tissue Res 235:551–559

    Article  PubMed  Google Scholar 

  19. Mackenzie IC, Dabelsteen E, Roed-Petersen B (1979) A method for studying epithelial-mesenchymal interactions in human oral mucosal lesions. Scand J Dent Res 87:234–243

    PubMed  Google Scholar 

  20. Martinez-Hernandez A, Amenta PS (1983) The basement membrane in pathology. Lab Invest 48:649–656

    Google Scholar 

  21. Moll R, Franke WW, Schiller DK, Geiger B, Krepler R (1982) The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells. Cell 31:11–24

    Article  PubMed  Google Scholar 

  22. Moll R, Franke WW, Volc-Platzer B, Krepler R (1982) Different keratin polypeptides in epidermis and other epithelia of human skin: a specific cytokeratin of molecular weight 46,000 in epithelia of the pilosebaceous tract and basal cell epitheliomas. J Cell Biol 95:285–295

    Article  PubMed  Google Scholar 

  23. Moll R, Krepler R, Franke WW (1983) Complex cytokeratin polypeptide patterns observed in certain human carcinomas. Differentiation 23:256–269

    PubMed  Google Scholar 

  24. Nelson DL, Little CK, Balian G (1983) Distribution of fibronectin and laminin in basal cell epitheliomas. J Invest Dermatol 80:446–452

    Article  PubMed  Google Scholar 

  25. Prunieras M, Regnier M, Fougere S, Woodley D (1983) Keratinocytes synthesize basal-lamina proteins in culture. J Invest Dermatol 81:74s-81s

    Article  PubMed  Google Scholar 

  26. Stanley JR, Beckwith JB, Fuller RP, Katz SI (1982) A specific antigenic defect of the basement membrane is found in basal cell carcinoma but not in other epidermal tumors. Cancer 50:1486–1490

    PubMed  Google Scholar 

  27. Sun T-T, Eicher R, Nelson WG, Tseng SCG, Weiss RA, Jarvinen M, Woodcock-Mitchell J (1983) Keratin classes: molecular markers for different types of epithelial differentiation. J Invest Dermatol 81:109s-115s

    Article  PubMed  Google Scholar 

  28. Van Cauwenberge D, Pierard GE, Foidart JM, Lapiere CM (1983) Immunohistochemical localization of laminin, type IV and type V collagen in basal cell carcinoma. Br J Dermatol 108:163–170

    PubMed  Google Scholar 

  29. Van Scott, EJ, Reinertson RP (1961) The modulating influence of stromal environment on epithelial cells studies in human autotransplants. J Invest Dermatol 36:109–131

    PubMed  Google Scholar 

  30. Viac J, Reano A, Thivolet J (1982) Cytokeratins in human basal and squamous cell carcinomas: biochemical, immunohistological findings and comparisons with normal epithelia. J Cutan Pathol 9:377–390

    PubMed  Google Scholar 

  31. Viac J, Reano A, Brochier J, Staquet MJ, Thivolet J (1983) Reactivity pattern of a monoclonal antikeratin antibody (KL1). J Invest Dermatol 81:351–354

    Article  PubMed  Google Scholar 

  32. Weber L, Krieg T, Muller PK, Kirsch E, Timpl R (1982) Immunofluorescent localization of type IV collagen and laminin in human skin and its application in junctional zone pathology. Br J Dermatol 106:267–273

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Institute of Pathology, University of Hamburg, Federal Republic of Germany

    T. H. Löning

  2. Dows Institute for Dental Research, The University of Iowa, USA

    I. C. Mackenzie

Authors
  1. T. H. Löning
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  2. I. C. Mackenzie
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Additional information

This study was supported by a grant from the Deutsche Forschungsgemeinschaft (Lo 285/2-2) and of the Hamburg Stiftung zur Förderung der Krebsbekämpfung (I 333), and the National Institutes of Health, NIDR RO1 DE05190

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Löning, T.H., Mackenzie, I.C. Immunohistochemical studies of basal cell carcinomas transplanted into nude mice. Arch Dermatol Res 279, 37–43 (1986). https://doi.org/10.1007/BF00404356

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  • DOI: https://doi.org/10.1007/BF00404356

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