Abstract
By using cytochrome-deficient mutants of Schwanniomyces castellii found previously, we measured the inhibition constants of azide and SHAM—alone or combined—for the different oxidative pathways, in order to determine the more suitable concentrations of inhibitors. This allowed us to measure the real capacity of each pathway. We calculated their affinity for oxygen, and determined that O2 was preferencially reduced by the cytochromic pathway, then by the SHAM-sensitive pathway, and finally by the SHAM+AA-insensitive pathway.
Similar content being viewed by others
References
Bahr JT & Bonner WD (1973) Cyanide insensitive respiration. II. Control of the alternate pathway. J. Biol. Chem 248: 3446–3450
De Troostemberg JC & Nyns EJ (1978) Kinetics of the respiration of cyanide-insensitive mitochondria from the yeast Saccharomycopsis lipolytica. Eur. J. Biochem. 85: 423–432
Dubreucq E, Boze Hélène, Fouilhé Martine, Moulin G & Galzy P (1990) Alternative pathways on Schwanniomyces castellii. I. Isolation and characterization of cytochrome-deficient mutants. Antonie van Leeuwenhoek 57: 123–130 (this issue)
Henry MF & Nyns EDJ (1975) Cyanide-insensible respiration. An alternative mitochondrial pathway. Sub. Cell. Biochem. 4: 1–65
Laties GG (1982) The cyanide-resistants, alternative path in higher plant respiration. Ann. Rev. Plant Physiol. 33: 519–555
Poinsot C, Moulin G, Claisse M & Galzy P (1987) Isolation and characterization of a mutant of Schwanniomyces castellii with altered respiration. Antonie van Leeuwenhoek 53: 65–70
Poinsot C, Boze Hélène, Moulin G & Galzy P (1986) Respiratory pathways in Schwanniomyces castellii. Biology of the Cell 58: 65–70
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Dubreucq, E., Boze, H., Moulin, G. et al. Alternative respiration pathways in Schwanniomyces castellii . Antonie van Leeuwenhoek 57, 131–137 (1990). https://doi.org/10.1007/BF00403947
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00403947