References
Prunieras M, Moreno G, Dosso Y, Vinzens F (1976) Studies on guinea pig skin cell cultures. V. Coculture of pigmented melanocytes and Albino keratinocytes, a model for the study of pigment transfer. Acta Derm Venereol (Stock) 56:1–9
Fritsch P, Tappeiner G, Kaaserer G (1979) Pure melanocyte cultures: Differential serum requirements of guinea pig keratinocytes and melanocytes in primary epidermal cell cultures. Arch Dermatol Res 265:181–187
Fritsch P, Tappeiner G, Huspek G (1981) Keratinocyte substrate adhesion is magnesium-dependent and calcium independent. Cell Biol Int Reports (in press)
Ishikawa H, Narimatsu H, Saito K (1973) Adjuvant effect of nystatin on in vitro antibody response of mouse spleen cells to heterologous erythrocytes. Cell Immunol 17:300–305
Kinsky SC (1970) Antibiotic interaction with model membranes. Ann Rev Pharmacol 10:119–128
Freedman JC, Hoffman JF (1979) Ionic and osmotic equilibria of human red blood cells treated with nystatin. J Gen Physiol 74:157–185
Oppenheimer SB, Potter RL, Barber ML (1973) Alteration of sea urchin embryo cell surface properties by mycostatin, a sterol-binding antibiotic. Dev Biol 33:218–223
Hatten ME, Burger MM (1979) Effect of polyene antibiotics on the lectin-induced agglutination of transformed and untransformed cell lines. Biochemistry 18:739–744
Dolberg D, Bissell MJ (1974) Side effect of amphotericin B-deoxycholate (fungizone) and nystatin in chick cells in culture. In Vitro 10:26–29
Crawford AC, Fettiplace R (1971) A method for altering the intracellular calcium concentration. J Physiol (Lond) 217:20P-21P
Peehl DM, Ham RG (1980) Clonal growth of human keratinocytes with small amounts of dialyzed serum. In Vitro 16:526–538
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Wilkins, L.M., Szabo, G. The establishment of pure mammalian epidermal melanocyte cultures through growth in high levels of mycostatin. Arch Dermatol Res 270, 483–486 (1981). https://doi.org/10.1007/BF00403793
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00403793