Summary
Ear skin of adult mice was used as a source of explant cultures of epidermal cells and the requirements for growth were defined. Two growth phases were observed, the first more rapid than the second. All-trans-retinoic acid or n-hexadecane was applied topically for up to 9 days and the skin was then used for explant cultures. During both treatments the ear became red and swollen. The epidermis became hyperkeratotic or deprived of its horny layer. The thickness of the stratum Malpighii increased to up to nine layers, from the normal of two or three layers. The stratum granulosum became multilayered and keratohyalin granules were abundant. Explants of n-hexadecane-treated skin behaved exactly as their normal controls. All-trans-retinoic acid induced an increase in migratory cell activity during the first growth phase. In the second growth phase the growth rate was similar or lower than that of the controls.
Zusammenfassung
Die Haut der Ohren von erwachsenen Mäusen war Material für Explantatkulturen von epidermalen Zellen und der Darstellung der Proliferationskinetik. Zwei Phasen des Wachstums wurden beobachtet, die erste ging schneller als die letztere vonstatten. All-trans-Retinoid Säure oder n-Hexadecan wurde am Ohr bis zu 9 Tagen gepinselt, dann wurde die Haut für Kulturen verwandt. Unter dieser Behandlung blieb das Ohr rot und angeschwollen. Die Epidermis wurde hyperkeratotisch und Stratum Corneum wurde abgestoßen. Die Dicke des Stratum Malpighii vermehrte sich bis zu neun Schichten, die Kontrollen um zwei bis drei Schichten. Das Stratum granulosum wurde vielschichtig und das Keratohyalin nahm reichlich zu. Die Explantate der Haut die mit n-Hexadecan behandelt wurden wie die der Kontrollen. Allotrans-Retinoid Säure induzierte eine vermehrte Migration von Zellen innerhalb der ersten Phase des Wachstums. Der Grad des Wachstums in der zweiten Phase war ähnlich oder geringer im Verhältnis zu den Kontrollen.
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Supported by the Karolinska Institutet, the Finsen and E. O. and Edla Johansson Foundations, the Swedish Psoriasis Association and the Swedish Medical Research Council (12x-5665)
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Hammar, H. Stimulated mouse ear epidermis in explant culture —The effect of retinoic acid and hexadecane. Arch Dermatol Res 270, 469–481 (1981). https://doi.org/10.1007/BF00403792
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DOI: https://doi.org/10.1007/BF00403792