Abstract
When microsomes from hypocotyls of Cucurbita pepo L. or coleoptiles of Zea mays L. were centrifuged on dextran-sucrose gradients a heterogeneity of auxin-accumulating vesicles was observed. Vesicles from the top part of the gradient showed saturable, specific accumulation of indole-3-acetic acid with only a small stimulation by phytotropins, and with very few binding sites for 1-N-naphthylphthalamic acid. In the vesicles from the lower part of the gradient, net accumulation of indole-3-acetic acid could be strongly increased by addition of phytotropins; binding of 1-N-naphthylphthalamic acid was high in this region. After two-phase partitioning, both kinds of vesicles were found in the upper-phase membrane fraction considered to be purified plasma membrane. The hypothesis is discussed that vesicles can be separated from the apical and basal parts of the cell's plasmalemma.
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Abbreviations
- CCO:
-
cytochrome-c oxidase
- CCR:
-
KCN-insensitive NADH-dependent cytochrome-c reductase
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- IAA:
-
indole-3-acetic acid
- IDPase:
-
inosine 5′-diphosphatase
- ION3:
-
ionophore mixture of carbonylcyanide-3-chlorophenylhydrazone, nigericin and valinomycin
- 1-NAA:
-
1-naphthaleneacetic acid
- NPA:
-
1-N-naphthylphthalamic acid
- PBA:
-
2-(1-pyrenoyl)benzoic acid
- UDPG:
-
uridine diphosphoglucose
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Lützelschwab, M., Asard, H., Ingold, U. et al. Heterogeneity of auxin-accumulating membrane vesicles from Cucurbita and Zea: a possible reflection of cell polarity. Planta 177, 304–311 (1989). https://doi.org/10.1007/BF00403587
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DOI: https://doi.org/10.1007/BF00403587