Abstract
Evidence is presented here for a close linkage between Akv-2, an ecotropic provirus found uniquely on chromosome 16 of AKR/N mice, and the immunoglobulin λ 1 light chain locus, Igl-1. No recombinants between the Igl-1 locus and Akv-2 were found by Southern blot analysis of DNA obtained from progeny of the backcross of (AKR/N × SJL/J)F1 to SJL/J, indicating that these genes map within 5.9 cM of each other. A probe specific for the flanking sequence of Akv-2 was used to detect the provirus, while one specific for the IgI-1 constant region was used to determine which allele of the structural gene was expressed in the backcross mice. The constant region of Igl-1 differs between AKR/N and SJL/J with respect to a site for the restriction endonuclease KpnI. This backcross was also used to seek recombinants between the regulatory, Igl-1r, and structural, Igl-1, loci of the immunoglobulin light chain locus, since the existence of such recombinants would prove that these loci are distinct. Since only parental types were recovered in the offspring, the structural and regulatory loci are no more than 2.3 cM apart, and the implications of this finding are discussed.
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Arp, B., McMullen, M. D., and Storb, U.: Sequences of immunoglobulin λ1 genes in a λ 1 defective mouse strain. Nature 298: 184–186, 1982
Brack, C., Hirama, M., Lenhard-Schuller, R., and Tonegawa, S.: A complete immunoglobulin gene is created by somatic recombination. Cell 15: 1–14, 1978
Buckler, C. E., Hoggan, M. D., Chan, H. W., Sears, J. F., Khan, A. S., Moore, J. L., Hartley, J. W., Rowe, W. P., and Martin, M. A.: Cloning and characterization of an envelope-specific probe from xenotropic murine leukemia proviral DNA. J. Virol. 41: 228–236, 1982a
Buckler, C. E., Staal, S. P., Rowe, W. P., and Martin, M. A.: Variation in the number of copies and in the genomic organization of ecotropic murine leukemia virus proviral sequences in sublines of AKR mice. J. Virol. 43: 629–640, 1982b
Chan, H. W., Bryon, T., Moore, J. L., Staal, S. P., Rowe, W. P., and Martin, M. A.: Identification of ecotropic proviral sequences in inbred mouse strains with a cloned subgenomic DNA fragment. Proc. Natl. Acad. Sci. U.S.A. 77: 5779–5783, 1980
Cory, S. and Adams, J.: Deletions are associated with somatic rearrangement of immunoglobulin heavy chain genes. Cell 19: 37–51, 1980
D'Eustachio, P., Bothwell, A. L. M., Takaro, T. K., Baltimore, D., and Ruddle, F. H.: Chromosomal locations of structural genes encoding murine immunoglobulin λ light chains. J. Exp. Med. 153: 793–800, 1981
Epstein, R., Lehmann, K., and Cohn, M.: Induction of λ 1-immunoglobulin is determined by a regulatory gene (rλ 1) linked (or identical) to the structural (cλ 1) gene. J. Exp. Med. 157: 1681–1686, 1983
Francke, U., Martinville, B., D'Eustachio, P., and Ruddle, F. H.: Comparative gene mapping: murine lambda light chain genes located in region cen→B5 of mouse chromosome 16 are non homologous to human chromosome 21. Cytogenet. Cell Genet. 33: 267–271, 1982
Geckeler, W., Faversham, J., and Cohn, M.: On a regulatory gene controlling the expression of the murine λ 1 light chain. J. Exp. Med. 148: 1122–1136, 1978
Kearney, F.J., Radbruch, A., Leisegang, B., and Rajewsky, K.: A new myeloma cell line that has lost immunoglobulin expression but permits the construction of antibody-secreting hybrid cell lines. J. Immunol. 123: 1548–1550, 1979
Kozak, A. and Rowe, W. P.: Genetic mapping of the ecotropic virus-inducing locus AKV-2 of the AKR mouse. J. Exp. Med. 152: 1419–1423, 1980
Lieberman, R. W., Humphrey, W., and Chien, C. C.: Identification and genetics of λ 1 1 light-chain allotype in the mouse. Immunogenetics 5: 515, 1977
Maniatis, T., Jeffrey, A., and Kleid, D. G.: Nucleotide sequence of the rightward operator of phage λ. Proc. Natl. Acad. Sci. U.S.A. 72: 1184–1188, 1975
Reth, M., Imanishi-Kari, T., and Rajewsky, K.: Analysis of the repertoire of anti-(4-hydroxy-3 nitrophenyl) acetyl (NP) antibodies in the C57B1/6 mice by cell fusion. II. Characterization of idiotopes by monoclonal anti-idiotope antibodies. Eur. J. Immunol. 9: 1004–1013, 1979
Riblet, R., Blomberg, B., Weigert, M., Lieberman, R., Taylor, B. A., and Potter, M.: Genetics of mouse antibodies: I. Linkage of the dextran response locus, VH Dex, to allotype. Eur. J. Immunol. 5: 775–777, 1975
Roderick, T., Davisson, M., and Lane, P.: Chromosome 16 and mahoganoid (md). Mouse News Letter 55: 18, 1976
Southern, E. M.: Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mal. Biol. 98: 503–517, 1975
Tabin, C. J., Bradley, S. M., Bargmann, C. I., Weinberg, R. A., Papageorge, A. G., Scolnier, E. M., Dhar, R., Lowy, D. R., and Chang, E. H.: Mechanism of activation of a human oncogene. Nature 300: 143–150, 1982
Takemori, T. and Rajewsky, K.: Lambda chain expression at different stages of ontogeny in C54B1/6, BALB/c and SJL mice. Eur. J. Immunol. 11: 618–625, 1981
Taylor, B. A., Bailey, D. W., Cherry, M., Riblet, R., and Weigert, M.: Genes for immunoglobulin heavy chain and serum prealbumin protein are linked in mouse. Nature 256: 644–646, 1975
Wahl, G. M., Stern, M., and Stark, G. R.: Efficient transfer of large DNA fragments from agorose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate. Proc. Natl. Acad. Sci. U.S.A. 16: 3683–3687, 1979
Weiss, S., Lehmann, K., and Cohn, M. Monoclonal antibodies to murine immunoglobulin isotypes. Hybridoma 2: 49–54, 1983
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Epstein, R., Lehmann, K., Cohnr, M. et al. Linkage of the Igl-1 structural and regulatory genes to Akv-2 on chromosome 16. Immunogenetics 19, 527–537 (1984). https://doi.org/10.1007/BF00403444
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DOI: https://doi.org/10.1007/BF00403444