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Rhythmisches Mycelwachstum bei Podospora anserina

VII. Der Einfluß oberflächenaktiver Substanzen und Antibiotica im Dunkeln und im Licht

Rhythmic mycelial growth in Podospora anserina

VII. The effect of surface-active substances and antibiotics in the dark and in the light

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Abstract

The correlation between membrane structure and morphogenesis in fungi was examined by studying the effect of surface-active and membrane-altering substances upon the colonial shape of Podospora anserina. The following results were obtained:

  1. 1.

    The alcohols 1-propanol, 1-butanol and 1-octanol inhibited hyphal elongation and thus caused zonations in the wild strain. In the clock-mutant zonata, however, the zonations disappeared at high concentrations of the alcohols.

  2. 2.

    The detergents sodium-dodecylsulfate, sodium-desoxycholate, triton X-100 and brij-35 also induced zonations, i.e. rhythmic growth. Growing on complete media, the sensibility of the strains used was markedly reduced.

  3. 3.

    The antibiotics nonactin, nigericin and monensin, too, inhibited growth and caused zonations, which, however, were light dependent. In contrast, valinomycin inhibited the elongation of the hyphae only.

The results are discussed as consequences of alterations in structure and features of the membranes.

Zusammenfassung

Zur Prüfung einer Wechselwirkung zwischen Membranstruktur und Morphogenese bei Pilzen wurde die Wirkung oberflächenaktiver und membranverändernder Substanzen auf die Wuchsform von Podospora anserina untersucht. Hierbei ergab sich folgendes:

  1. 1.

    Die Alkohole 1-Propanol, 1-Butanol und 1-Octanol hemmten das Wachstum des Wildstammes und induzierten dabei Zonierungen. Bei der “clock”-Mutante zonata hingegen wurden die Zonierungen unterdrückt.

  2. 2.

    Die Detergenzien Natrium-dodecylsulfat, Natrium-desoxycholat, Triton X-100 und Brij-35 führten ebenfalls zu zoniertem, d. h. rhythmischem Wuchs. Auf Komplettmedium zeigten die benutzten Stämme geringere Empfindlichkeit gegen diese Substanzen als auf Minimalmedien.

  3. 3.

    Auch die Antibiotica Monensin, Nigericin und Nonactin riefen die mit einer Wuchshemmung verbundenen Zonierungen hervor, die jedoch lichtabhängig waren. Valinomycin dagegen hemmte nur das Hyphenwachstum.

In der Diskussion wird versucht, die Ergebnisse auf Veränderungen der Membranen und ihrer Eigenschaften zurückzuführen.

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Literatur

  • Bartnicki-Garcia, S., Lippman, E.: Fungal morphogenesis. Cell wall construction in Mucor rouxii. Science 165, 302–304 (1969)

    Google Scholar 

  • Biedermann, M.: Einwirkung von Detergenzien auf die Thylakoide von Rhodospirillum rubrum. Arch. Mikrobiol. 75, 171–178 (1971)

    Google Scholar 

  • Bornefeld, T., Lysek, G.: Rhythmic mycelial growth in Podospora anserina. V. The levels of phosphorylated intermediates. Arch. Mikrobiol. 87, 119–128 (1972)

    Google Scholar 

  • Bünning, E., Moser, I.: Influence of valinomycin on circadian leaf movements of Phaseolus. Proc. nat. Acad. Sci. (Wash.) 69, 2732–2733 (1972)

    Google Scholar 

  • Engelmann, D. M., Terry, T. M., Morowitz, H. J.: Characterization of the plasma membrane of Mycoplasma laidlawii. Biochim. biophys. Acta. (Amst.) 135, 381–390 (1967)

    Google Scholar 

  • Esser, K.: An introduction to Podospora anserina. Neurospora Newsletter 15, 27–31 (1969a)

    Google Scholar 

  • Esser, K.: The influence of pH on rhythmic mycelial growth in Podospora anserina. Mycologia (N.Y.) 61, 1008–1011 (1969b)

    Google Scholar 

  • Faraj-Salman, A.-G.: Zur Induktion einer endogenen Rhythmik bei Mutanten des Pilzes Penicillium claviforme Bainier. I. Wirkungsweise von Alkoholen. Arch. Protistenk. 113, 306–313 (1971)

    Google Scholar 

  • Hall, M. P.: An analysis of the factors controlling the growth form of certain fungi, with special reference to Sclerotinia (Monilia) fructigena. Ann. Bot. 47, 538–578 (1933)

    Google Scholar 

  • Hall, J. D., Crane, F. L.: Fractionation of mitochondrial membranes with sodium deoxycholate. Biochim. biophys. Acta (Amst.) 255, 602–619 (1972)

    Google Scholar 

  • Lanyi, J. K.: Influence of electron transport on the interaction between membrane lipids and triton X-100 in Halobacterium cutirubrum. Biochemistry 12, 1433–1438 (1973)

    Google Scholar 

  • Lombardi, F. J., Reeves, J. P., Kaback, H. R.: Mechanisms of active transport in isolated bacterial membrane vesicles. XIII. Valinomycin-induced rubidium transport. J. biol. Chem. 248, 3551–3565 (1973)

    Google Scholar 

  • Lysek, G.: Rhythmic mycelial growth in Podospora anserina. III. Effect of metabolic inhibitors. Arch. Mikrobiol. 78, 330–340 (1971)

    Google Scholar 

  • Lysek, G.: Rhythmisches Mycelwachstum bei Podospora anserina. IV. Rhythmischer Verlauf der Trockengewichtsproduktion im flüssigen Medium. Arch. Mikrobiol. 81, 221–233 (1972a)

    Google Scholar 

  • Lysek, G.: Rhythmic mycelial growth in Podospora anserina. VI. An attempt to elucidate the growth pattern of a clock mutant. Arch. Mikrobiol. 87, 129–137 (1972b)

    Google Scholar 

  • Lysek, G.: A unified model of rhythmic growth in fungi. (In Vorbereitung)

  • Lysek, G., Esser, K.: Rhythmic mycelial growth in Podospora anserina. II. Evidence for a correlation with carbohydrate metabolism. Arch. Mikrobiol. 75, 360–371 (1971)

    Google Scholar 

  • Packer, L., Murakami, S., Mehard, C. W.: Ion transport in chloroplasts and plant mitochondria. Ann. Rev. Plant Physiol. 21, 271–304 (1970)

    Google Scholar 

  • Philippot, J.: Studies of human red blood cell membrane using sodium deoxycholate. I. Mechanism of the solubilization. Biochim. biophys. Acta (Amst.) 225, 201–213 (1971)

    Google Scholar 

  • Schnaitman, C. A.: Solubilization of the cytoplasmic membrane of Escherichia coli by triton X-100. J. Bact. 108, 545–552 (1971a)

    Google Scholar 

  • Schnaitman, C. A.: Effect of ethylenediaminetetraacetic acid, triton X-100, and lysozyme on the morphology and chemical composition of isolated cell walls of Escherichia coli. J. Bact. 108, 553–563 (1971b)

    Google Scholar 

  • Tavlitzki, J.: Sur la croissance de Podospora anserina en milieu synthétique. C. R. Acad. Sci. (Paris) 238, 2341–2343 (1954)

    Google Scholar 

  • Trinci, A. P. J., Collinge, A.: Influence of L-Sorbose on the growth and morphology of Neurospora crassa. J. gen. Microbiol. 78, 179–192 (1973)

    Google Scholar 

  • Woldringh, C. L., Van Iterson, W.: Effects of treatment with sodium dodecyl sulfate on the ultrastructure of Escherichia coli. J. Bact. 111, 801–813 (1972)

    Google Scholar 

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Authors and Affiliations

  1. Botanisches Institut der Technischen Universität München in Freising-Weihenstephan, Freising-Weihenstephan, Deutschland

    G. Lysek & H. v. Witsch

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  1. G. Lysek
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  2. H. v. Witsch
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Herrn Prof. Dr. M. Steiner zum 70. geburtstag gewidmet.

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Lysek, G., Witsch, H.v. Rhythmisches Mycelwachstum bei Podospora anserina . Arch. Microbiol. 97, 227–237 (1974). https://doi.org/10.1007/BF00403062

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  • DOI: https://doi.org/10.1007/BF00403062

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