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Spermidine and flower-bud differentiation in thin-layer explants of tobacco

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Abstract

Three lines of evidence indicate a connection between high spermidine levels and floral initiation in thin-layer tissue cultures of Wisconsin-38 tobacco (Nicotiana tabacum L.). (1) Spermidine levels are much higher in floral buds than in vegetative buds. (2) Inhibition of spermidine synthesis by cyclohexylamine prevents the rise in spermidine titer, inhibits floral initiation and promotes the formation of vegetative buds instead. (3) Application of exogenous spermidine causes floral initiation in cultures which would otherwise form vegetative buds.

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References

  • Batchelor, K.W., Smith, R.A., Watson, N.S. (1986) Dicyclohexylamine is not an inhibitor of spermidine synthase. Biochem. J., 233, 307–308

    Google Scholar 

  • Birecka, H., Garraway, M.O., Baumann, R.J., McCann, P.P. (1986) Inhibition of ornithine decarboxylase and growth of the fungus Helminthosporium maydis. Plant Physiol. 80, 798–800

    Google Scholar 

  • Bradford, M.M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248–254

    Google Scholar 

  • Feirer, R.P., Mignon, G., Litvay, J.D. (1984) Arginine decarboxylase and polyamines required for embryogenesis in the wild carrot. Science 223, 1433–1435

    Google Scholar 

  • Feirer, R.P., Wann, S.R., Einspahr, D.W. (1985) The effects of spermidine synthesis inhibitors on in-vitro plant development. Plant Growth Reg. 3, 319–327

    Google Scholar 

  • Heby, O. (1981) Role of polyamines in the control of cell proliferation and differentiation. Differentiation 19, 1–20

    Google Scholar 

  • Kaur-Sawhney, R., Shekhawat, N.S., Galston, A.W. (1985) Polyamine levels as related to growth, differentiation and senescence in protoplast-derived cultures of Vigna aconitifolia and Avena sativa. Plant Growth Reg. 3, 329–337

    Google Scholar 

  • Malmberg, R.L., McIndoo, J. (1983) Abnormal floral development of a tobacco mutant with elevated polyamine levels. Nature 305, 623–625

    Google Scholar 

  • Martin-Tanguy, J. (1985) The occurrence and possible function of hydroxycinnamoyl acid amides in plants. Plant Growth Reg. 3, 381–399

    Google Scholar 

  • Murashige, T., Skoog, F. (1962) A revised medium for rapid growth and biossays with tobacco tissue cultures. Physiol. Plant. 15, 473–497

    Google Scholar 

  • Rajam, M.V., Galston, A.W. (1985) The effects of some polyamine biosynthetic inhibitors on growth and morphology of photopathogenic fungi. Plant Cell Physiol. 26, 683–692

    Google Scholar 

  • Sindhu, R.K., Cohen, S.S. (1984) Propylamine transferases in Chinese cabbage leaves. Plant Physiol. 74, 645–649

    Google Scholar 

  • Slocum, R.D., Kaur-Sawhney, R., Galston, A.W. (1984) The physiology and biochemistry of polyamines in plants. Arch. Biochem. Biophys. 235, 283–303

    Google Scholar 

  • Tabor, C.W., Tabor, H. (1985) Polyamines in microorganisms. Microbiol. Rev. 49, 81–99

    Google Scholar 

  • Tiburcio, A.F., Kaur-Sawhney, R., Ingersoll, R.B., Galston, A.W. (1985) Correlation between polyamines and pyrrolidine alkaloids in developing tobacco callus. Plant Physiol. 78, 323–326

    Google Scholar 

  • Tran Thanh Van K. (1973a) In vitro control of de novo flower bud, root and callus differentiation from excised epidermal tissues. Nature 246, 44–45

    Google Scholar 

  • Tran Than Van, K. (1973b) Direct flower neoformation from superficial tissues of small explant of Nicotiana tabacum L. Planta 115, 87–92

    Google Scholar 

  • Vince-Prue, D. (1975) Photoperodism in plants. McGraw Hill Co., New York

    Google Scholar 

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Kaur-Sawhney, R., Tiburcio, A.F. & Galston, A.W. Spermidine and flower-bud differentiation in thin-layer explants of tobacco. Planta 173, 282–284 (1988). https://doi.org/10.1007/BF00403022

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  • DOI: https://doi.org/10.1007/BF00403022

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