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An unusually high number of direct repeats detected by sequence analysis of the dispersed EcoRI-family fragments in Lupinus luteus L.

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Abstract

The Lupinus luteus genome contains a highly repetitive fraction of sequences named the EcoRI family. Two EcoRI molecules, 1071 and 1079 base pairs in length, were cloned, sequenced and compared. Analysis of the internal-sequence organization revealed a number of short direct repeats. Their involvement in the formation of the EcoRI-family fragments is postulated. Evidence is presented for the dispersed type of genomic organization of the EcoRI-family fragments.

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Abbreviations

AluI, BspRI, EcoRI, Mbo, PstI:

restriction nucleases

bp:

base pair

G, A, T, C:

deoxynucleotides: dGMP, dAMP, dTMP and dCMP

pBR322 and pUC18:

plasmids used as cloning vehicles

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Authors and Affiliations

  1. Institute of Physiology and Biochemistry, Department of Biochemistry, Medical Academy of Łódź, ul. Lindleya 6, 90-131, Łódź, Poland

    T. Sakowicz, G. Gałązka, A. Konarzewska, M. Kwinkowski & J. Kłysik

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  1. T. Sakowicz
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  2. G. Gałązka
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  3. A. Konarzewska
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  4. M. Kwinkowski
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  5. J. Kłysik
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Sakowicz, T., Gałązka, G., Konarzewska, A. et al. An unusually high number of direct repeats detected by sequence analysis of the dispersed EcoRI-family fragments in Lupinus luteus L.. Planta 168, 207–213 (1986). https://doi.org/10.1007/BF00402965

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  • DOI: https://doi.org/10.1007/BF00402965

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