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Internode length in Pisum sativum L. The kinetics of growth and [3H]gibberellin A20 metabolism in genotype na Le

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Abstract

The relationship between shoot growth and [3H]gibberellin A20 (GA20) metabolism was investigated in the GA-deficient genotype of peas, na Le. [17-13C, 3H2]gibberellin A20 was applied to the shoot apex and its metabolic fate examined by gas chromatographic-mass spectrometric analysis of extracts of the shoot and root tissues. As reported before, [13C, 3H2]GA1, [13C, 3H2]GA8 and [13C, 3H2]GA29 constituted the major metabolites of [13C, 3H2]GA20 present in the shoot. None of these GAs showed any dilution by endogenous 12C-material. [13C, 3H2]GA29-catabolite was also a prominent metabolite in the shoot tissue but showed pronounced isotope dilution probably due to carry-over of endogenous [12C]GA29-catabolite from the mature seed. In marked contrast to the shoot tissue, the two major metabolites present in the roots were identified as [13C, 3H2]GA8-catabolite and [13C, 3H2]GA29-catabolite. Both of these compounds showed strong dilution by endogenous 12C-material. Only low levels of [13C, 3H2]GA1, [13C, 3H2]GA8, [13C, 3H2]GA20 and [13C, 3H2]GA29 accumulated in the roots. It is suggested that compartmentation of GA-catabolism may occur in the root tissue in an analogous manner to that shown in the testa of developing seeds. Changes in the levels of [1β,3α-3H2]GA20 metabolites over 10 d following application of the substrate to the shoot apex of genotype na Le confirmed the accumulation of [3H]GA-catabolites in the root tissues. No evidence was obtained for catabolic loss of [3H]GA20 by complete oxidation or conversion to a methanol-inextractable form. The results indicate that the root system may play an important role in the regulation of biologically active GA levels in the developing shoot of Na genotypes of peas.

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Abbreviations

GAn :

gibberellin An

GC-MS:

gas chromatography-mass spectrometry

HPLC:

high-pressure liquid chromatography

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Authors and Affiliations

  1. School of Chemistry, University of Bristol, BS8 1TS, Bristol, UK

    Timothy J. Ingram & Jake MacMillan

  2. Department of Botany, University of Tasmania, 7001, Hobart, Tasmania, Australia

    James B. Reid

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  1. Timothy J. Ingram
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  2. James B. Reid
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  3. Jake MacMillan
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Ingram, T.J., Reid, J.B. & MacMillan, J. Internode length in Pisum sativum L. The kinetics of growth and [3H]gibberellin A20 metabolism in genotype na Le . Planta 164, 429–438 (1985). https://doi.org/10.1007/BF00402957

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