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Correlation between the presence of membrane-bound auxin binding and root regeneration in cultured tobacco cells

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Abstract

When cell-suspension cultures and callus tissue from Nicotiana tabacum are grown on medium containing α-naphthaleneacetic acid (NAA) and kinetin, three classes of auxin-binding proteins can be detected. When the herbicide 2,4-dichlorophenoxyacetic acid is used instead of both NAA and kinetin, one of these sites, which is membranebound, disappears. After retransferring cells to medium containing NAA and kinetin, this membrane-bound site reappears after four to eight weeks. This reappearance is correlated with the ability of the cells to regenerate roots.

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Abbreviations

IAA:

indole-3-acetic acid

NAA:

α-naphthaleneacetic acid

2,4-D:

2,4-dichlorophenoxyacetic acid

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Authors and Affiliations

  1. Chicago Medical School, University of Health Sciences, 3333 Green Bay Road, 60064, North Chicago, Il, USA

    A. C. Maan

  2. Department of Plant Molecular Biology, Botanisch Laboratorium der Rijksuniversiteit, Nonnensteeg 3, NL-2311, VJ Leiden, The Netherlands

    P. C. G. van der Linde, P. A. A. Harkes & K. R. Libbenga

Authors
  1. A. C. Maan
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  2. P. C. G. van der Linde
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  3. P. A. A. Harkes
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  4. K. R. Libbenga
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Maan, A.C., van der Linde, P.C.G., Harkes, P.A.A. et al. Correlation between the presence of membrane-bound auxin binding and root regeneration in cultured tobacco cells. Planta 164, 376–378 (1985). https://doi.org/10.1007/BF00402949

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  • DOI: https://doi.org/10.1007/BF00402949

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