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Uptake of [14C]sucrose in isolated minor-vein networks of Commelina benghalensis L

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Abstract

Maceration with pectinase (4.5h) of Commelina benghalensis L. leaves stripped at either side yielded isolated vein networks consisting of four to five secondary veins and tertiary cross veins (=minor veins). Examination with Evans Blue and injection of Fluorescein F showed that 80% of the veins were viable. Proof of normal functioning of isolated minor veins was that [14C]sucrose fed to an apical vein network attached to the remaining intact part of the leaf was absorbed and finally arrived in the petiole. Sucrose uptake by veins obeyed Michaelis-Menten kinetics (K m 5·10-4 mol l-1; V max (light) 3.2 μmol h-1 g-1 fresh weight, V max (dark) 1.5 μmol h-1 g-1 fresh weight). A linear component, not inhibited by carbonylcyanide m-chlorophenylhydrazone and p-chloromercuribenzenesulfonic acid, was present. Maximal uptake took place at 5 mmol l-1 K+; concentrations of K+ higher than 10 mmol l-1 decreased the rate of uptake. The uptake rates by isolated veins and veins in situ (in disks) were in the same order of magnitude. Altogether, isolated veins promise to be a useful system for the study of loading.

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Abbreviations

CCCP:

carbonylcyanide m-chlorophenylhydrazone

EDTA:

ethylenediamine tetraacetic acid

PCMBS:

p-chloromercuribenzenesulfonic acid

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Authors and Affiliations

  1. Department of Plant Physiology, Rijksuniversiteit Utrecht, Lange Nieuwstraat 106, NL-3512 PN, Utrecht, The Netherlands

    A. J. E. val Bel & A. J. Koops

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  1. A. J. E. val Bel
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  2. A. J. Koops
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val Bel, A.J.E., Koops, A.J. Uptake of [14C]sucrose in isolated minor-vein networks of Commelina benghalensis L. Planta 164, 362–369 (1985). https://doi.org/10.1007/BF00402947

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  • DOI: https://doi.org/10.1007/BF00402947

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