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A comparative immunocytochemical localization study of phosphoenolpyruvate carboxylase in leaves of higher plants

Planta volume 155, pages 24–30 (1982)Cite this article

Abstract

The intracellular localization of phosphoenolpyruvate (PEP) carboxylase in plants belonging to the C4, Crassulacean acid metabolism (CAM) and C3 types was invetigated using an immunocytochemical method with an immune serum raised against the sorghum leaf enzyme. The plants studied were sorghum, maize (C4 type), kalanchoe (CAM type), french bean, and spinach (C3 type). In the green leaves of C4 plants, it was shown that the carboxylase was located in the mesophyll and stomatic cells, being largely cytosolic in the mesophyll cells. Similarly, in CAM plants, the enzyme was found mainly outside the chloroplasts. In contrast, in C3 plants, the PEP carboxylase appeared to be distributed between the cytosol and the chloroplasts of foliar parenchyma. Examination of sections from etiolated leaves showed fluorescence emission from etioplasts and cytosol for the parenchyma of french bean as well as for the bundle sheath and mesophyll of sorghum leaves. This data indicated that during the greening process photoregulation and evolution of PEP carboxylase is dependent on the tissue and on the metabolic type of the plant considered.

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Abbreviations

CAM:

Crassulacean acid metabolism

PEP:

phosphoenolpyruvate

References

  • Coons, A.H., Kaplan, M.H. (1950) Localization of antigen in tissue cells. Improvements in method for detection of antigen by means of fluorescent antibody. J. Exp. Med. 91, 1–13

    Article  PubMed  Google Scholar 

  • Francis, K. (1979) Photosynthesis by isolated chloroplasts of Sorghum vulgare. Experientia 35, 1324–1326

    PubMed  Google Scholar 

  • Gadal, P., Vidal, J., Perrot, C., Godbillon, G., Burlet, A., Bouville, J. (1980) Influence of light on phosphoenolpyruvate carboxylase in Sorghum leaves: Immunochemical study. Fifth international congress on Photosynthesis (in press)

  • Graham, T.A., Gunning, B.E.S. (1970) Localization of legumin and vicilin in bean cotyledon cells using fluorescent antibodies. Nature (London) 288, 81–82

    Google Scholar 

  • Hatch, M.D., Slack, C.R. (1970) Photosynthetic CO2-fixation pathways. Annu. Rev. Plant Physiol. 21, 141–162

    Google Scholar 

  • Jones, R.L., Ru-Fang Chen (1976) Immunohistochemical localization of α-amylase in barley aleurone cells. J. Cell Sci. 20, 183–198

    PubMed  Google Scholar 

  • Kagawa, T., Hatch, M.D. (1974) Light dependent metabolism of carbon compounds by mesophyll chloroplasts from plants with the C4 pathway of photosynthesis. Aust. J. Plant Physiol. 1, 51–64

    Google Scholar 

  • Kornberg, H.L. (1966) Anaplerotic sequences and their role in metabolism. In: Essays in biochemistry, vol. 2, pp. 1–31, Campbell, P.N., Greville, G.D., eds. Academic Press, London

    Google Scholar 

  • Mayor, M.D., Hampton, J.C., Rosario, B. (1961) A simple method for removing the resin from epoxy-embedded tissue. J. Biophys. Biochem. 9, 909–910

    Google Scholar 

  • Nishimura, M., Akazawa, T. (1974) Studies on spinach leaf ribulosebiphosphate carboxylase. Carboxylase and oxygenase reaction examined by immunochemical method. Biochemistry 13, 2277–2281

    PubMed  Google Scholar 

  • Perrot, C., Vidal, J., Burlet, A., Gadal, P. (1981) On the cellular localization of phosphoenolpyruvate carboxylase. Planta 151, 226–231

    Google Scholar 

  • Queiroz, O., Morel, C. (1974) Photoperiodism and enzyme activity. Towards a model for the control of circadian metabolic rhythms in the Crassulacean acid metabolism. Plant Physiol. 53, 596–602

    Google Scholar 

  • Rathnam, C.K.M., Das, V.S.R. (1975) Inter and intracellular distribution of carbonic anhydrase, PEP carboxylase and RUDP carboxylase in leaves of Eleusine coracana, a C4 plant. Z. Pflanzenphysiol. 75, 360–364

    Google Scholar 

  • Schnarrenberger, C., Gross, D., Burkhard, C., Herbert, M. (1980) Cell organelles from Crassulacean acid metabolism (CAM) plants. Planta 147, 477–484

    Google Scholar 

  • Scopes, R.K. (1974) Measurements of protein by spectrophotometry at 205 nm. Anal. Biochem. 59, 277–282

    PubMed  Google Scholar 

  • Slack, C.R., Hatch, M.D. (1967) Comparative studies on the activity of carboxylases and other enzymes in relation to the new pathway of photosynthetic carbon dioxide fixation in tropical grasses. Biochem. J. 103, 660–665

    PubMed  Google Scholar 

  • Slack, C.R., Hatch, M.D., Goodchild, D.J. (1969) Distribution of enzymes in mesophyll and parenchyma-sheath chloroplasts of Maize leaves in relation to the C4 dicarboxylic acid pathway of photosynthesis. Biochem. J 114, 489–498

    PubMed  Google Scholar 

  • Stulen, I., Oaks, A. (1977) Asparagine synthetase in corn roots. Plant Physiol. 60, 680–683

    Google Scholar 

  • Sutton, B.G., Osomond, C.B. (1972) Dark fixation of CO2 by crassulacean plants. Evidence for a single carboxylation step. Plant Physiol. 50, 360–365

    Google Scholar 

  • Ting, I.P., Osmond, C.B. (1973) Photosynthetic phosphoenolpyruvate carboxylase. Plant Physiol. 51, 439–442

    Google Scholar 

  • Vidal, J., Cavalie, G. (1974) Mise en évidence de formes isofonctionnelles de la PEP carboxylase chez Phaseolus vulgaris L. Physiol. Veg. 12, 175–188

    Google Scholar 

  • Vidal, J., Cavalie, G., Gadal, P. (1976) Etude de la phosphoénolpyruvate carboxylase du Haricot et du Sorgho par électrophorèse sur gel de polyacrylamide. Plant Sci. Lett. 7, 265–270

    Google Scholar 

  • Vidal, J., Godbillon, G., Gadal, P. (1980) Recovery of active, highly purified phosphoenolpyruvate carboxylase from specific immunoadsorbent column. FEBS Lett. 118, 31–34

    Google Scholar 

  • Willmer, C., Kanai, R., Pallas, J.E. Jr., Black, C.C. Jr. (1973) Detection of high levels of phosphoenolpyruvate carboxylase in leaf epidermal tissue and its significance in stomatal movements. Life Sci. 12, 151–155

    Google Scholar 

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Affiliations

  1. Laboratoire de Physiologie Végétale Métabolique, ERA CNRS no 799, Université de Paris Sud, Centre d'Orsay, F-91405, Orsay-Cedex, France

    C. Perrot-Rechenmann, J. Vidal, J. Brulfert, A. Burlet & P. Gadal

  2. Laboratoire Phytotron CNRS, F-91190, Gif-sur-Yvette, France

    J. Brulfert

  3. Laboratoire d'Histologie A, Faculté de Médecine, B.P. 184, F-54505, Vandœuvre les Nancy-Cedex, France

    A. Burlet

Authors
  1. C. Perrot-Rechenmann
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  2. J. Vidal
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  3. J. Brulfert
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  4. A. Burlet
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  5. P. Gadal
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Perrot-Rechenmann, C., Vidal, J., Brulfert, J. et al. A comparative immunocytochemical localization study of phosphoenolpyruvate carboxylase in leaves of higher plants. Planta 155, 24–30 (1982). https://doi.org/10.1007/BF00402927

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  • DOI: https://doi.org/10.1007/BF00402927

Key words

  • C3-, C4-plant
  • Crassulacean acid metabolism
  • Phosphoenolpyruvate carboxylase
  • Photosynthesis, C3 and C4