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Evidence for a cytosolic-dependent light induction of chloroplastic glutamine synthetase during greening of etiolated rice leaves

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Abstract

During the greening of etiolated rice leaves, total glutamine synthetase activity increases about twofold, and after 48 h the level of activity usually observed in green leaves is obtained. A density-labeling experiment with deuterium demonstrates that the increase in enzyme activity is due to a synthesis of the enzyme. The enhanced activity obtained upon greening is the result of two different phenomena: there is a fivefold increase of chloroplastic glutamine synthetase content accompanied by a concommitant decrease (twofold) of the cytosolic glutamine synthetase. The increase of chloroplastic glutamine synthetase (GS2) is only inhibited by cycloheximide and not by lincomycin. This result indicates a cytosolic synthesis of GS2. The synthesis of GS2 was confirmed by a quantification of the protein by an immunochemical method. It was demonstrated that GS2 protein content in green leaves is fivefold higher than in etiolated leaves.

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Abbreviations

AbH:

heavy chain of antibodies

AbL:

light chain of antibodies

AP:

acid phosphatase

CH:

cycloheximide

G6PDH:

glucose-6-phosphate dehydrogenase

GS:

glutamine synthetase

GS1 :

cytosolic glutamine synthetase

GS2 :

chloroplastic glutamine synthetase

LC:

lincomycin

NAD-MDH:

NAD malate dehydrogenase

NADP-G3PDH:

NADP glyceraldehyde-3-phosphate dehydrogenase

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Authors and Affiliations

  1. Laboratoire de Physiologie Végétale Métabolique, ERA CNRS no 799, Bâtiment 430, Université de Paris Sud, Centre d'Orsay, F-91405, Orsay-Cedex, France

    Bertrand Hirel, Jean Vidal & Pierre Gadal

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  1. Bertrand Hirel
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  2. Jean Vidal
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  3. Pierre Gadal
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Hirel, B., Vidal, J. & Gadal, P. Evidence for a cytosolic-dependent light induction of chloroplastic glutamine synthetase during greening of etiolated rice leaves. Planta 155, 17–23 (1982). https://doi.org/10.1007/BF00402926

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  • DOI: https://doi.org/10.1007/BF00402926

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