Abstract
Photodynamic therapy (PDT) consists of the combination of photosensitizers absorbing light mainly in the red spectral region and irradiation with light of corresponding wavelengths. We analysed its effects on the cytokine secretion (IL-1Β, TNFα, IL-6) of freshly isolated peripheral mononuclear cells from six patients with chronic plaque-stage psoriasis in comparison with PUVA. PUVA treatment resulted in a decreased production of all three cytokines, but most pronounced in the case of IL-6. PDT caused a similar change in the cytokine pattern, but its effectiveness was lower. In vivo fluorescence recordings were performed on psoriatic plaque lesions after topical application of the photosensitizer Photosan-3. Under irradiation, progressive photobleaching was noted with increasing radiation dosage. This is the first reported study of photochemical reactions using on-line fluorescence recordings during PDT of psoriatic lesions in vivo. Our results demonstrate the capacity of PDT to cause immunomodulatory effects similar to PUVA, thus indicating its potential application to the treatment of this common disease.
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References
Bos JD (1988) The pathomechanisms of psoriasis; the skin immune system and cyclosporin. Br J Dermatol 118: 141–155
Dougherty TJ (1986) Photosensitization of malignant tumours. Semin Surg Oncol 2: 24–37
ElsÄsser-Beile U, von Kleist S, StÄhle W, Schurhammer-Fuhrmann C, Schulte Mönting J, Gallati H (1993) Cytokine levels in whole blood cell cultures as parameters of the cellular immunologic activity in patients with malignant melanoma and basal cell carcinoma. Cancer 71: 231–236
Gupta AK, Anderson TF (1987) Psoralen photochemotherapy. J Am Acad Dermatol 17: 703–734
Johnston RB (1988) Monocytes and macrophages. N Engl J Med 318: 747–752
Kessel D (1986) Sites of photosensitization by derivates of hematoporphyrin. Photochem Photobiol 44: 489–494
König K, Wabnitz H, Dietel W (1990) Variation in the fluorescence decay properties of haematoporphyrin derivative durings its conversion to photoproducts. J Photochem Photobiol [B] 8: 103–111
König K, Rück A, Schneckenburger H (1992) Fluorescence detection and photodynamic activity of endogenous protoporphyrin in human skin. Opt Eng 31: 1470–1474
Kostron H, Bellnier DA, Lin CW, Swarz MR, Martuza RL (1986) Distribution, retention and phototoxicity of hematoporphyrin derivate in a rat glioma. J Neurosurg 64: 768–774
McCaughan JS (1990) Photodynamic therapy of skjn and esophageal cancers. Cancer Invest 8: 407–416
Musser DA, Fiel RJ (1991) Cutaneous photosensitizing and immunosuppressive effects of a series of tumour localizing porphyrins. Photochem Photobiol 53: 119–123
Neuner P, Charvat B, Knobler R, Urbanski A, Luger TA, Schwarz T (1992) PUVA treatment downregulates the release of TNF alpha, IL-1 beta and IL-8 by peripheral blood mononuclear cells (abstract). J invest Dermatol 98: 505
Parrish JA, Fitzpatrick TB, Tanebaum L, Pathak MA (1974) Photochemotherapy of psoriasis with oral methoxsalen and longwave ultraviolet light. N Engl J Med 291: 1207–1211
Pathak MA (1984) Mechanisms of psoralen photosensitization reactions. Natl Cancer Inst Monogr 66: 41–46
Ragaz A, Ackermann B (1979) Evolution, motivation and regression of lesions of psoriasis. Am J Dermatopathol 1: 199–214
Weishaupt KR, Gomer CJ, Dougherty TJ (1976) Identification of singlet oxygen as the cytotoxic agent in the photoinactivation of a murine tumour. Cancer Res 36: 2322–2329
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Boehncke, W.H., König, K., Kaufmann, R. et al. Photodynamic therapy in psoriasis: suppression of cytokine production in vitro and recording of fluorescence modification during treatment in vivo. Arch Dermatol Res 286, 300–303 (1994). https://doi.org/10.1007/BF00402219
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DOI: https://doi.org/10.1007/BF00402219