Abstract
Mutations affecting pigmentation of the cyanobacterium Synechocystis sp. 6701 were induced with ultraviolet light. Two mutants with phycobilisome structural changes were selected for structural studies. One mutant, UV08, was defective in chromatic adaptation and incorporated phycoerythrin into phycobilisomes in white or red light at a level typical of growth in green light. The other mutant, UV16, was defective in phycobilisome assembly: little phycocyanin was made and none was attached to the phycobilisome cores. The cores were completely free of any rod substructures and contained the major core peptides plus the 27,000 Mr linker peptide that attaches rods to the core. Micrographs of the core particles established their structural details. Phycoerythrin in UV 16 was assembled into rod structures that were not associated with core material or phycocyanin. The 30,500 Mr and 31,500 Mr linker peptides were present in the phycoerythrin rods with the 30,500 Mr protein as the major component. Phycobilisome assembly in vivo is discussed in light of this unusual mutant.
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Abbreviations
- PE:
-
phycoerythrin
- PC:
-
phycocyanin
- AP:
-
allophycocyanin
- W:
-
white light
- G:
-
green light
- R:
-
red light
- SDS:
-
sodium dodecyl sulfate
- Na−K−PO4 :
-
equimolar solutions of NaH2PO4 · H2O and K2HPO4 · 3 H2O titrated to the desired pH
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Anderson, L.K., Rayner, M.C. & Eiserling, F.A. Ultra-violet mutagenesis of Synechocystis sp. 6701: mutations in chromatic adaptation and phycobilisome assembly. Arch. Microbiol. 138, 237–243 (1984). https://doi.org/10.1007/BF00402128
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DOI: https://doi.org/10.1007/BF00402128