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“Activated” monocytes in gastric cancer patients

I. Increased Fc receptor expression, antibody-dependent cellular cytotoxicity and NBT reduction

  • Original Papers
  • Experimental Oncology
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Summary

The Fc receptor expression, antibody-dependent cellular cytotoxicity (ADCC), and nitro-blue tetrazolium (NBT) reduction of peripheral blood monocytes from 150 patients with different stages of gastric cancer was assessed and compared with results obtained in 77 normal persons and 104 patients with non-malignant diseases of the gut. Monocytes of cancer patients showed an increased ability to form rosettes with human 0, Rh+ erythrocytes coated with D-specific antibody. ADCC and NBT reduction were also elevated but no correlation was found with the stage of disease. However, all these phenomena were related to the tumor load as elevated values were the same 4–6 months after surgery in the unresectable-tumor group, while they decreased in patients with resectable tumors. These observations suggest that monocytes of some cancer patients are functionally altered (“activated”) in the course of disease.

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Abbreviations

AC:

adherent cells

ADCC:

antibody-dependent cellular cytoxicity

51Cr:

sodium chromate

EAhu :

antibody-coated human erythrocytes

EAhu-RFC:

cells forming rosettes with EAhu

FCS:

foetal calf serum

MEM:

minimum essential medium

NBT:

nitroblue tetrazolium

NR:

neutral red

NSE:

non-specific esterase

O.D.:

optical density

PBM:

peripheral blood mononuclear cells

References

  • Boyum A (1968) Isolation of mononuclear cells and granulocytes from human blood. Scand J Clin Lab Invest 21:77–99

    PubMed  Google Scholar 

  • Cohn ZA (1978) The activity of mononuclear phagocytes: fact, fancy and future. J Immunol 121:813–816

    PubMed  Google Scholar 

  • Eccles SA (1978) Macrophages and cancer. In: Castro JE (ed) Immunological aspects of cancer. MPT Press Lancaster (pp 123–154)

    Google Scholar 

  • Evans R, Alexander P (1972) Role of macrophages in tumor immunity. 1. Co-operation between macrophages and lymphoid cells in syngeneic tumor immunity. Immunology 23:615–621

    PubMed  Google Scholar 

  • Fidler IJ (1978) Recognition and destruction of target cells by tumoricidal macrophages. Isr J Med Sci 14:177–191

    PubMed  Google Scholar 

  • Hausman MS, Brosman S, Snyderman R, Mickey MR, Fahey J (1975) Defective monocyte function in patients with genitourinary carcinoma. J Natl Cancer Inst 55:1047–1053

    PubMed  Google Scholar 

  • Hedley DW, Currie GA (1978) Monocytes and macrophages in malignant melanoma. III. Reduction of NBT by peripheral blood monocytes. Br J Cancer 37:747–752

    PubMed  Google Scholar 

  • Jerrells RT, Dean JH, Richardson G, Cannon GB, Herberman RB (1979) Increased monocyte-mediated cytostasis of lymphoid cell lines in breast and lung cancer patients. Int J Cancer 23:768–776

    PubMed  Google Scholar 

  • Kay AB, McVie JG (1977) Monocyte chemotaxis in bronchial carcinoma and cigarette smokers. Br J Cancer 36:461–466

    PubMed  Google Scholar 

  • Keller R (1976) Promotion of tumor growth in vivo by antimacrophage agents. J Natl Cancer Inst 57:1355–1361

    PubMed  Google Scholar 

  • Kjedsberg CR, Pay GD (1978) A qualitative and quantitative study of macrophages in patients with malignant solid tumors. Cancer 41:2236–2241

    PubMed  Google Scholar 

  • McVie JG, Logan ECM, Kay AB (1977) Monocyte function in cancer patients. Eur J Cancer 13: 351–353

    PubMed  Google Scholar 

  • Nathan CF, Murray HW, Cohn ZA (1980) The macrophage as an effector cell. N Eng J Med 303: 622–626

    Google Scholar 

  • Norris DA, Morris RM, Sanderson RJ, Kohler PF (1979) Isolation of functional subsets of human peripheral blood monocytes. J Immunol 123:166–172

    PubMed  Google Scholar 

  • Nyholm RE, Currie GA (1978) Monocytes and macrophages in malignant melanoma. II. Lysis of antibody-coated human erythrocytes as an assay of monocyte function. Br J Cancer 37:337–344

    PubMed  Google Scholar 

  • Rhodes J (1977) Altered expression of human monocyte Fc receptor in malignant disease. Nature 265:253–255

    PubMed  Google Scholar 

  • Rhodes J, Bishop M, Benfield J (1979) Tumor serveillance: how tumor may resist macrophage-mediated host defense. Science 203:179–182

    PubMed  Google Scholar 

  • Snyderman R, Pike MC (1976) Defective macrophage migration produced by neoplasm: identification of an inhibitor of macrophage chemotaxis. In: Fink M (ed) The macrophage in neoplasia. Academic Press New York, London (pp 49–67)

    Google Scholar 

  • Tucker SB, Pierre RV, Jordon RE (1977) Rapid identification of monocytes in a mixed mononuclear cell fraction. J Immunol Methods 14:266–269

    Article  Google Scholar 

  • Uracz W, Pituch A, Mytar B, Noworolski J, Zembala M (1978) Monocyte changes in cancer patients and their role in mitogen-induced lymphocyte responses. Arch Immunol Ther Exp 26:428–432

    Google Scholar 

  • Weston WL, Dustin RD, Hecht SK (1975) Quantitative assays of human monocyte function. J. Immunol Methods 8:213–221

    Article  PubMed  Google Scholar 

  • Wood GW, Neff JE, Stephens R (1979) Relationship between monocytosis and T-lymphocyte function in human cancer. J Natl Cancer Inst 63:587–592

    PubMed  Google Scholar 

  • Zembala M, Lemmel EM, Uracz W (1980) Activation of human monocytes for NBT reduction and the suppression of lymphocyte response to mitogens. Clin Exp Immunol 41:309–316

    PubMed  Google Scholar 

  • Zembala M, Lemmel EM (1980) Inhibitory factor/s of lymphoproliferation produced by synovial fluid mononuclear cells from rheumatoid arthritis patients: the role of monocytes in suppression. J Immunol 125:1087–1092

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Division of Clinical Immunology, Institute of Paediatrics, Wielicka 265, 30-663, Cracow, Poland

    Wojciech Uracz, Anna Pituch-Noworolska & Marek Zembala

  2. First Surgical Department, Copernicus Medical School, Cracow, Poland

    Tadeusz Popiela & Antoni Czupryna

Authors
  1. Wojciech Uracz
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  2. Anna Pituch-Noworolska
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  3. Marek Zembala
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  4. Tadeusz Popiela
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  5. Antoni Czupryna
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Uracz, W., Pituch-Noworolska, A., Zembala, M. et al. “Activated” monocytes in gastric cancer patients. J Cancer Res Clin Oncol 104, 181–190 (1982). https://doi.org/10.1007/BF00402066

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  • DOI: https://doi.org/10.1007/BF00402066

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