Summary
Female wistar rats were fed on an Mg-deficient diet. After 8 to 10 weeks, the thymus glands were strongly degenerated and the number of lymphocytes was reduced, especially in the cortex. Thymus degeneration was associated with a decreased rate of DNA, RNA, and protein biosynthesis and necrosis and phagocytosis of lymphocytes. The degeneration was normalized after feeding on an Mg-rich diet. After 10 or 11 weeks of Mg deficiency, local cell proliferations of immature lymphocytes with a great number of free ribosomes were found in some thymus glands. The local cell proliferations developed into infiltrating tumors without metastases. In lymphoma cells the Na+ and Ca2+ content, the turnover of cellular Na+ and K+, and the aerobic production of CO2 and lactate were increased.
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References
Alcock NW, Shils ME (1974) Serum immunoglobuline G in the magnesium depleted rat. Proc Soc Exp Biol Med 145:855–858
Alcock NW, Shils ME, Lieberman PH, Erlandson RA (1973) Thymic changes in the magnesium depleted rat. Cancer Res 33:2196–2204
Alcock NW, Shils ME, Lieberman PH, Erlandson RA (1976) Thymic changes in magnesium depleted rats and a relationship to dietary calcium levels. II. Internat Symposium on Magnesium, Montreal abstracts, p 23
Averdunk R, Günther T (1980a) Effect of concanavalin A on intracellular K+ and Na+ concentration and K+ transport of human lymphocytes. Immunobiology 38:132–144
Averdunk R, Günther T (1980b) Characterization of the concanavalin A induced increase in lymphocyte cell membrane permeability by furosemide. Immunobiology 38:358–364
Averdunk R, Ostapovicz B, Günther T (1975) Die Rolle von cyclischem AMP und Ca bei der Permeabilitätsänderung Mg-arm gewachsener Tumorzellen. Z klin Chem klin Biochem 13:361–366
Battifora H (1971) Effects of magnesium deficiency on blood cells. Clinical and experimental data. In: Durlach J (ed) I. International Symposium on Magnesium Deficit in Human Pathology. Vittel (p 501)
Bois P, Sandborn EB, Messier PE (1969) A study of thymic lymphosarcoma developing in magnesium deficient rats. Cancer Res 29:763–775
Bossi D, Cittadini A, Wolf F, Milani A, Magalini S, Terranova T (1979) Intracellular calcium and magnesium content and aerobic lactate production in intact Ehrlich ascites tumor cells. FEBS Letters 104:6–12
Cameron IL, Smith NKR, Pool TB, Sparks RL (1980) Intracellular concentration of sodium and other elements as related to mitogenesis and oncogenesis in vivo. Cancer Res 40:1493–1500
Carpentieri U, Sordahl LA (1980) Respiratory and calcium transport functions of mitochondria isolated from normal and transformed human lymphocytes. Cancer Res 40:221–224
Durant S, Homo F, Duval D (1980) Calcium and A 23187-induced cytolysis of mouse thymocytes. Biochem Biophys Res Comm 93:385–391
Elin RJ (1975) The effect of magnesium deficiency in mice on serum immunoglobulin concentrations and antibody plaque-forming cells. Proc Soc Exp Biol Med 148:620–624
Fraker PJ, de Pasquale-Jardieu P, Zwickl CM, Luecke RW (1978) Regeneration of T-cell helper function in zinc-deficient adult mice. Proc Nat Acad Sci 75:5660–5664
Goldstein G, Lau C (1980) Thymopoietin and Immunoregulation. In: Beers RF Jr, Bassett EG (eds) Polypeptide hormones. Raven Press, New York, p 459
Goldstein AL, Low TLK, Thurman GB (1980) Thymosin: Basic properties and clinical application in the treatment of immunodeficiency diseases and cancer. In: Beers RF Jr, Bassett EG (eds) Polypeptide hormones. Raven Press New York, p 449
Günther T, Averdunk R (1970) K+-Transport und Stoffwechsel von Mg arm gewachsenen Yoshida-Ascitestumorzellen. Z klin Chem klin Biochem 8: 621–625
Günther T, Averdunk R (1976) Wirkungen des “Ca-Ionophors” X-537 A auf den Elektrolytgehalt und Stoffwechsel von Yoshida-Ascites-Tumorzellen: Ein Modell zur Analyse des Mg-Mangels. J Clin Chem Clin Biochem 14:365–371
Günther T, Averdunk R (1979) Reduced lectin stimulation of lymphocytes from magnesium-deficient rats. J Clin Chem Clin Biochem 17:51–55
Iwata T, Incefy GS, Tanaka T, Fernandes G, Menendez-Botet CJ (1978) Low levels of serum thymic factor [FTS] in zinc-deficient A/Jax mice. Fed Proc 37:1827
Johnson MA, Weber MJ (1980) Serum stimulation of potassium fluxes, ouabain binding, and sodium fluxes in quiescent chicken embryo fibroblasts. J Cell Physiol 103:363–370
Kaiser N, Edelman IS (1978) Calcium dependence of ionophore A 23187-induced lymphocyte cytotoxicity. Cancer Res 38:3599–3603
Lengle EE, Gustin NC, Gonzalez F, Menahan LA, Kemp RG (1978) Energy metabolism in thymic lymphocytes of normal and leukemic AKR mice. Cancer Res 38:1113–1119
McKeehan WL, Ham RG (1978) Calcium and magnesium ions and the regulation of multiplication in normal and transformed cells. Nature 275:756–758
Metcalf D (1966) The thymus. Springer, Berlin Heidlberg New York
Potter M (1976) Tumors of immunoglobulin producing cells and thymus derived lymphocytes. In: Melchers F., Rajewsky K (eds) The immune system. 27. Mosbacher Colloquium. Springer, Berlin Heidelberg New York, p 141
Racker E (1976) Why do tumor cells have a high aerobic glycolysis? J Cell Physiol 89:697–700
Rayssiguier Y, Larvor P, Augusti Y, Durlach J (1977) Serum proteins in magnesium-deficient rat Ann Biol Anim Bioch Biophys 17:147–152
Schilling K, Börnig H, Cumme G, Hoppe H (1980) Effect of magnesium-dependent cell membrane alterations on the transport of K+ in Ehrlich ascites tumor cells. Acta Biol Med Germ 39:177–184
Schmalbeck J, Willems WR, Dorn F, Günther T (1972) Über den Lipidstoffwechsel im Magnesium-Mangel. Z klin Chem klin Biochem 10:270–274
Shen SS, Hamamoto ST, Bern HA, Steinhardt RA (1978) Alteration of sodium transport in mouse mammary epithelium associated with neoplastic transformation. Cancer Res 38:1356–1361
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Averdunk, R., Bippus, P.H., Günther, T. et al. Development and properties of malignant lymphoma induced by magnesium deficiency in rats. J Cancer Res Clin Oncol 104, 63–73 (1982). https://doi.org/10.1007/BF00402054
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DOI: https://doi.org/10.1007/BF00402054