Abstract
The relationship between the xylose induced decrease in hexokinase PII activity and the derepression of invertase synthesis in yeast is described. When xylose was added to cells growing in a chemostat under nitrogen limitation, the catabolic repression was supressed as shown by the large increase on invertase levels even if glucose remained high. The glucose phosphorylating-enzymes were separated by hydroxylapatite chromatography and it is shown that the treatment with xylose is accompanied by a loss of 98% hexokinase PII and a 50% of the PI isoenzyme, whereas the levels of glucokinase as well as those of glucose-6-phosphate, fructose-6-phosphate, pyruvate and ATP remained unaffected.
The analysis of the enzymes present in cells grown in ethanol, limiting glucose and high glucose, shows that hexokinase PII predominates in cells under catabolic repression, the opposite is true for glucokinase, whereas hexokinase PI remains unaffected.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Barnard EA (1975) Hexokinases from yeast. In: Wood WA (ed) Methods in enzimology, vol 42. Academic Press. New York, pp 6–20
Entian KD (1981) A carbon catabolite repression mutant of Saccharomyces cerevisiae with elevated hexokinase activity: Evidence for regulatory control of hexokinase PII synthesis. Mol Gen Genet 184:278–282
Entian KD, Mecke D (1982) Genetic evidence for a role of hexokinase isozyme PII in carbon catabolite repression in Saccharomyces cerevisiae. J Biol Chem 257:870–874
Entian KD, Zimmermann FK (1980) Glycolytic enzymes and intermediates in carbon catabolite repression mutants of Saccharomyces cerevisiae. Mol Gen Genet 117:345–350
Gascón S, Lampen JO (1968) Purification of the internal invertase of yeast. J Biol Chem 243:1567–1572
Gascón S, Ottolenghi P (1972) Influence of glucose concentration of the medium on the invertase content of a strain of Saccharomyces bearing the SUC2 gene. C R Trab Lab Carlsberg 39:15–24
Gilliland RB (1969) The raffinose fermentation of Saccharomyces pastorianus and Saccharomyces bayanus. Antonie van Leeuwenhoek. J Microbiol Serol 35:13–23
Holzer H (1976) Catabolite inactivation in yeast. Trends Biochem Sci 1:178–180
Kaplan NO, Ciotti MM (1957) Enzymatic determination of ethanol. In: Colowick SA, Kaplan NO (eds) Methods in enzymology, vol 3. Academic Press, New York, pp 252–255
Lobo Z, Maitra PK (1977) Genetics of yeast hexokinase. Genetics 86:727–744
Michels CA, Romanowski A (1980) Pleiotropic glucose repressionresistant mutation in Saccharomyces carlsbergensis. J Bacteriol 143:674–679
Nelson NJ (1957) Colorimetric analysis of sugars. In: Collowick SP, Kaplan NO (eds) Methods in enzymology, vol 3. Academic Press, New York, pp 85–86
Sáez MJ, Lagunas R (1976) Determination of intermediary metabolites in yeast. Critical examination of the effect of sampling conditions and recommendations for obtaining true levels. Mol Cell Biochem 13:73–78
Zimmermann FK, Scheel I (1977) Mutants of Saccharomyces cerevisiae resistant to carbon catabolite repression. Mol Gen Genet 154:75–82
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Fernández, R., Herrero, P., Gascón, S. et al. Xylose induced decrease in hexokinase PII activity confers resistance to carbon catabolite repression of invertase synthesis in Saccharomyces carlsbergensis . Arch. Microbiol. 139, 139–142 (1984). https://doi.org/10.1007/BF00401988
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00401988