Summary
We describe the regulatory properties of two strains carrying either the ilvA624 or the ilvA625 mutations, located in the structural gene for threonine deaminase. Crude extracts of both these strains possess a threonine deaminase activity migrating on polyacrylamide gels, differently from the wild type enzyme. Growth studies demonstrate that these mutations do not cause a limitation of isoleucine biosynthesis, suggesting normal catalytic activity of deaminase.
A regulatory consequence of the ilvA624 allele is a derepression of the isoleucine-valine biosynthetic enzymes, which is recessive to an ilvA + allele. The ilvA625 mutation causes a derepression which is dominant in an ilvA625/ilvA + diploid. We interpret these data assuming that threonine deaminase, previously shown to be an autogenous regulator of the ilv genes, lacks a repressor function in the ilvA624 mutant, while in the ilvA625 mutant it is a better activator than wild type threonine deaminase.
The data are discussed in terms of a model requiring that threonine deaminase, or a precursor of it, is in equilibrium between two forms, one being an activator of gene expression and the other being a repressor.
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Guardiola, J., Cervone, F., Lamberti, A. et al. Dual autogenous regulatory role of threonine deaminase in Escherichia coli K-12. Molec. Gen. Genet. 159, 27–32 (1978). https://doi.org/10.1007/BF00401744
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DOI: https://doi.org/10.1007/BF00401744