Abstract
Optimization procedures for the reversed-phase separations of six phthalates using the isoselective multisolvent gradient elution (IMGE) system are described. A systematic experimental design has been employed to gather retention data on the compounds in a mixture. The data were then fitted into a second-order polynomial equation and an overlapping resolution mapping (ORM) technique of data analysis was subsequently used to establish the optimum solvent mixture for the highest resolution of all adjacent peaks in the chromatogram.
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References
Berridge, J. C.: 1985, Techniques for the Automated Optimization of HPLC Separations', Wiley, Chichester, pp. 70–94.
Cerbulis, J., Ard, J. S.: 1967, ‘Isolation and detection of dioctyl phthalate from milk lipids’, J. Assoc. Offic. Anal. Chem., 50, 646–650.
Ishida, M., Suyama, K. and Ard, J. S.: 1980, ‘Background contamination by phthalates commonly encountered in the chromatographic analysis of lipid samples’, J. Chromatogr., 189, 421–424.
Ishida, M., Suyama, K. and Adachi, S.: 1981, ‘Occurrence of dibutyl and di (2-ethylhexyl)phthalate in chicken eggs’, J. Agric. Food Chem., 29, 72–74.
Jaeger, R. J. and Rubin, R. J.: 1972, ‘Migration of a phthalate ester plasticizer from polyvinyl chloride blood bags into stored human blood and its localization in human tissues’, N. Engl. J. Med., 287, 1114–1118.
Kirkland, J. J. and Glajch, J. L.: 1983, ‘Optimization of mobile phases for multisolvent gradient elution liquid chromatography’, J. Chromatogr., 255, 27–39.
Khan, M. R., Ong, C. P., Li, S. F. Y. and Lee, H. K.: 1990, ‘Optimization of the isocratic HPLC separation of selected phthalates using the overlapping resolution mapping technique’, J. Chromatogr., 513, 360–366.
Mayer, F. L. Jr., Stalling, D. L. and Johnson, J. L.: 1972, ‘Phthalate esters as environmental contaminants’, Nature (London), 238, 411–413.
Snyder, L. R.: 1974, ‘Classification of the solvent properties of common liquids’, J. Chromatogr., 92, 223–230.
Snyder, L. R., Dolan, J. W. and Grant, J. R.: 1979, ‘Gradient elution in high performance liquid chromatography. I. Theoretical basis for reversed phase system’, J. Chromatogr., 165, 3–30.
Williams, D. T.: 1973, ‘Dibutyl-and di(2-ethylhexyl)phthalate in fish’, J. Agric. Food Chem., 21, 1128–1129.
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Yao, Y.J., Khan, M.R., Ong, C.P. et al. Optimization of the gradient HPLC separation of selected phthalates using the overlapping resolution mapping technique. Environ Monit Assess 19, 83–91 (1991). https://doi.org/10.1007/BF00401300
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DOI: https://doi.org/10.1007/BF00401300